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Wasrin Syafii
Department of Forest Products, Faculty of Forestry and Environment, IPB University

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Anti-Termite Activity of Melia azedarach Extracts Yanico Hadi Prayogo; Romi Irka Putra; Izza Firdausi Hadiyanto; Evie Nihayah; Wasrin Syafii; Rita Kartika Sari; Irmanida Batubara
Jurnal Sylva Lestari Vol. 10 No. 1 (2022): January
Publisher : Department of Forestry, Faculty of Agriculture, University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jsl.v10i1.549

Abstract

Natural preservatives have been widely considered as non-synthetic materials that can prevent the biodegradation of wood due to subterranean termite attacks, namely Coptotermes curvignathus. Melia azedarach is a fast-growing species that has been widely studied for its antifeedant compounds and shows good potential to be developed as a biopesticide, especially as an anti-termite. This study aimed to identify the termicide and antifeedant properties of the wood, leaves, and bark extracts of Melia azedarach against subterranean termites and identify their bioactive compounds. The acetone extract from the three parts of the tree was fractionated. The most active fractions were selected from each part based on the percentage of termite mortality and antifeedant activity and analyzed for its bioactive compounds by pyrolysis GCMS. The ethyl acetate fraction of Melia azedarach bark exhibited strong toxic properties compared to other fractions. In the leaf section, the diethyl ether fraction showed the best termite repellent (antifeedant) properties. The anti-termite properties influenced phenolic compounds and organic acid that dominated the bark ethyl acetate fraction and leaf diethyl ether fraction. These findings show the great potential of bioactive compounds from biomaterials to be developed as a promising biopesticide. Keywords: anti-termite, bioactive compound, Melia azedarach, natural preservative
Ultrasound-Assisted Extraction of Antimalarial Compounds from the Bidara Laut (Strychnos ligustrina) Heartwood Sri Familasari; Rita Kartika Sari; Wasrin Syafii; Anne Carolina; Umi Cahyaningsih; Siti Sa'diah
Jurnal Sylva Lestari Vol. 11 No. 3 (2023): September
Publisher : Department of Forestry, Faculty of Agriculture, University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jsl.v11i3.682

Abstract

The aqueous wood extract of bidara laut (Strychnos ligustrina) from maceration demonstrated the greatest antimalarial activity against Plasmodium falciparum strain 3D7. However, this method has several limitations, including a lengthy extraction time, which allows for microorganism contamination. The aim of this study was to quantify the yield, evaluate the antimalarial effectiveness of the P. falciparum growth in-vitro, and analyze the aqueous bidara laut heartwood extracts phytochemical composition from the two different extraction methods,  namely the method of maceration extraction for 24 hours and the ultrasound-assisted extraction (UAE) for 30 (UAE30), 45 (UAE45), and 60 (UAE60) minutes. The bidara laut heartwood extract from the UAE60 method had relatively similar yield and antimalarial activity with the bidara laut heartwood extract from the UAE45 method. However, these extracts had higher yield and antimalarial activity than the extracts from the maceration and the UAE30 methods. The results of LC-MS/MS analysis revealed that the main compounds in the UAE45 and UAE60 extracts were strychnine, quinaprilat, and 3,4-bis-cyclobut-3-ene-1,2-dione, which has potent antimalarial properties. Keywords: antimalarial activity, Plasmodium falciparum, quinaprilat, strychnine, Strychnos ligustrina
Chemical Constituent of Acacia auriculiformis Wood Extractives and Their Antioxidant Activity Yanico Hadi Prayogo; Irmanida Batubara; Rita Kartika Sari; Saat Egra; Kosei Yamauchi; Tohru Mitsunaga; Wasrin Syafii
Jurnal Sylva Lestari Vol. 11 No. 3 (2023): September
Publisher : Department of Forestry, Faculty of Agriculture, University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jsl.v11i3.753

Abstract

Acacia auriculiformis showed good potential to be developed as a raw material for bioactive compounds. The heartwood of A. auriculiformis is still rarely explored. The purpose of this study was to identify the bioactive components of the heartwood. Six compounds were identified from the heartwood through nuclear magnetic resonance and mass spectroscopy, namely the C-3,4',7,8 hydroxyl substituted flavonoids and chalcone. Two methylated teracacidins and chalcone-like teracacidin were identified for the first time in the A. auriculiformis heartwood, along with three known compounds. These compounds showed good radical scavenging and reduction activity, compared to crude extract and ascorbic acid, using three different antioxidant assays. The molecular structure-dependent activity was observed to affect the trend of the different antioxidant activities. This finding shows good potential for further development of plant parts of A. auriculiformis from Indonesia as new raw materials for medicines. Keywords: Acacia auriculiformis, antioxidant, chalcone, flavonoid, heartwood