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All Journal The Journal of Experimental Life Sciences (JELS) The Journal of Pure and Applied Chemistry Research
Widodo Widodo
Brawijaya University
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience

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 1 
Synthesis and Characterization of Cellulose Acetate and Nanocellulose Acetate from Sengon Agroindustrial Waste (Paraserianthes falcataria) Nur Ikhtiarini; Masruri MASRURI; Siti Mariyah Ulfa; Widodo Widodo
The Journal of Pure and Applied Chemistry Research Vol 11, No 3 (2022): Edition September-December 2022
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2022.011.03.644

Abstract

Synthesis and characterization of cellulose acetate and nanocellulose acetate have been carried out. Cellulose was isolated from sengon wood powder waste using 6% NaOH solution and bleached with NaOCl. The cellulose was hydrolyzed with 60% formic acid to obtain nanocellulose. Cellulose and nanocellulose were then modified with acetic anhydride. The synthesized products were characterized by FTIR to identify organic functional groups, powder XRD to measure crystallinity, and SEM to observe surface morphology. Solubility tests in several types of solvents were also performed. The degree of substitution (DS) of cellulose acetate and nanocellulose acetate was carried out based on Indonesia’s SNI 0444:2009 method. The DS calculation show that the cellulose acetate and nanocellulose have DS of 2.9 and 2.1, respectively.
In Silico Study to Predict the Potential of Beta Asarone, Methyl Piperonylketone, Coumaric Acid in Piper Crocatum as Anticancer Agents Ahmed Hasan Abkar; Moch. Sasmito Djati; Widodo Widodo
The Journal of Experimental Life Science Vol. 11 No. 3 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.03.04

Abstract

Piper Crocatum Ruiz & Pav leaves often be used to treat various diseases, including cancer, empirically. This study aimed to analyze the anticancer activities of Piper Crocatum bioactive compounds via In Silico analysis. The methods were biological activity analysis, cell line cytotoxicity activity, SwissADME, STITCH, molecular docking, and molecular dynamics simulation. The investigated bioactive compounds were β-asarone, methyl piperonylketone, and coumaric acid. The results showed the biological activities of the compounds related to anticancer were anti-mutagenic, TNF expression inhibitive, and MMP9 expression inhibitive. The prediction of cytotoxicity analysis results showed that investigated bioactive compounds were toxic on various tumor cell lines. Based on swissADME results, almost all compounds have good pharmacological properties, except coumaric acid. Docking analysis demonstrated the presence of bioactive compounds inhibited TNFa, HER2, and MMP9 as the target protein. The molecular dynamic result was confirmed using molecular dynamic and it shows that β-asarone interaction was stable against MMP9 and TNF protein showed by the low RMSD value. The study found the investigated bioactive compounds of Piper crocatum have the activity of anti-cancer via the inhibition of TNFa and MMP9 protein. However, further research still needs to be done to confirm the prediction results of this In Silico study. Keywords: anticancer, In Silico, molecular docking, Piper crocatum.
Protein Structure and Function Analysis Method of Aminoacyl-tRNA Synthetase Cofactor and Biotinylation Effect: Journal Review Restu Nugraha; Chien Chia Wang; Widodo Widodo
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1083.411 KB) | DOI: 10.21776/ub.jels.2019.009.01.10

Abstract

Protein has been known as an important macromolecule which has a vital role among the living organism. One of the most interesting protein is Arc1p, which is a yeast-specific tRNA-binding protein. Arc1p is a unique protein that has the ability to form a ternary complex with glutamyl-tRNA synthetase (GluRSc) and methionyl-tRNA synthetase (MetRS) in the cytoplasm. This complex can significantly enhance the aminoacylation efficiency of these two aaRSs to their respective cognate tRNAs. Recently, it was found that Arc1p can be biotinylated via post-translational modification at Lys86 (K86) in the N-domain. Here, we try to figure it out what kind of method that will help to create some clear information both in structure and function of this protein, when mutations occur inside of the K86 site within SSKD motifs of Arc1p. Several methods to better understanding obviously about protein characteristics comprises protein structural analysis; such as gel mobility shift assay, CD Spectroscopy, and limited proteolysis; protein functional analysis, and in silico modeling.Keywords: Arc1p, biotinylation, function, in silico, structure.
Co-Authors Ahmed Hasan Abkar Chien Chia Wang Masruri Masruri Moch Sasmito Djati Nur Ikhtiarini Restu Nugraha Siti Mariyah Ulfa