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PERTUMBUHAN BAKTERI Pseudomonas aeruginosa DAN DEKOLORISASI SENYAWA PEWARNA STRAWBERRY RED DAN ORANGE YELLOW DALAM KONDISI CURAH Lukito, Antonius Budi Darmawan; Goretti, Maria; Goeltom, Mangihot Tua
CALYPTRA : Jurnal Ilmiah Mahasiswa Universitas Surabaya Vol 2, No 1 (2013): CALYPTRA : Jurnal Ilmiah Mahasiswa Universitas Surabaya
Publisher : University of Surabaya

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Dewasa ini, limbah pewarnaan tekstil memiliki masalah dalampengolahannya terutama dalam pendegradasian warna. Konsentrasi pewarnadidalam setiap limbah berbeda-beda. Oleh karena itu, dilakukan penelitian untukpertumbuhan bakteri Pseudomonas aeuriginosa sebagai bakteri pendegradasiwarna di dalam media alternatif serta dekolorisasi senyawa pewarna tekstil.Pewarna yang digunakan dalam percobaan ini merupakan pewarna artifisial yangbiasanya digunakan pada pewarnaan tekstil yaitu Strawberry Red dan OrangeYellow. Dari hasil penelitian terhadap dua media alternatif (air rendaman kedelaidan Nutrient Broth) didapatkan bahwa media alternatif yang optimum untukdigunakan adalah Nutrient Broth 75%, sedangkan bakteri Pseudomonasaeruginosa memiliki kemampuan dekolorisasi yang paling optimum padapewarna Strawberry Red 300 ppm dan pewarna Orange Yellow 100 ppm. Untuklaju pertumbuhan bakteri Pseudomonas aeruginosa pada berbagai konsentrasipewarna, tidak diperoleh laju pertumbuhan spesifik yang berbeda signifikan.

Immobilization of Lipase on Surfactant-Modified Bentonite and Its Application for Biodiesel Production from Simulated Waste Cooking Oil Chrisnasari, Ruth; Yonardi, Angelina; Lie, Hesti; Widi, Restu Kartiko; Purwanto, Maria Goretti Marianti
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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The influence of bentonite modification by cationic surfactant hexadecyl-trimethyl-ammonium bromide (HDTMA-Br) and tetramethyl ammonium hydroxide (TMAOH) on its capability to immobilize lipase was studied. Modification of bentonite was conducted by the adding of 4-6% (v/v) HDTMA-Br and TMAOH respectively. The obtained immobilized lipases then were characterized to observe the optimum pH and temperature as well as their stability during reuse application. The observed results show that there is no significant difference between the variations of HDTMA-Br concentrations to the percentage of immobilized enzyme which can immobilize lipase up to 75-78%. However, the best concentration of TMAOH is 4% (v/v) which can immobilize lipase up to 97.95%. The obtained immobilized lipases on HDTMA-Br-modified bentonite show the optimum catalytic activity on reaction temperature of 35-40 oC and pH of 7.5. In other hand, the optimum catalytic activity of immobilized lipases on TMAOH-modified bentonite is 40oC of incubation temperature and pH of 7. The immobilized lipases on both HDTMA-Br and TMAOH modified bentonite are stable enough so it could be re-used four times before its activity decreased by 48,565% and 46.83 % respectively. Keywords: Lipase, Cationic Surfactant, Bentonite, HDTMA-Br, TMAOH, Biodiesel, Immobilization.

Immobilization of Lipase on Surfactant-Modified Bentonite and Its Application for Biodiesel Production from Simulated Waste Cooking Oil Ruth Chrisnasari; Angelina Yonardi; Hesti Lie; Restu Kartiko Widi; Maria Goretti Marianti Purwanto
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

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The influence of bentonite modification by cationic surfactant hexadecyl-trimethyl-ammonium bromide (HDTMA-Br) and tetramethyl ammonium hydroxide (TMAOH) on its capability to immobilize lipase was studied. Modification of bentonite was conducted by the adding of 4-6% (v/v) HDTMA-Br and TMAOH respectively. The obtained immobilized lipases then were characterized to observe the optimum pH and temperature as well as their stability during reuse application. The observed results show that there is no significant difference between the variations of HDTMA-Br concentrations to the percentage of immobilized enzyme which can immobilize lipase up to 75-78%. However, the best concentration of TMAOH is 4% (v/v) which can immobilize lipase up to 97.95%. The obtained immobilized lipases on HDTMA-Br-modified bentonite show the optimum catalytic activity on reaction temperature of 35-40 oC and pH of 7.5. In other hand, the optimum catalytic activity of immobilized lipases on TMAOH-modified bentonite is 40oC of incubation temperature and pH of 7. The immobilized lipases on both HDTMA-Br and TMAOH modified bentonite are stable enough so it could be re-used four times before its activity decreased by 48,565% and 46.83 % respectively. Keywords: Lipase, Cationic Surfactant, Bentonite, HDTMA-Br, TMAOH, Biodiesel, Immobilization.

Character Improvement of Red Rice (Oryza Sativa L.) Cv. Barak Cenana by Mutagenesis using Gamma Irradiation Aloysia Sri Pujiyanti; Bintang Kerta Wijaya; Ida Bagus Made Artadana; Popy Hartatie Hardjo; Maria Goretti Marianti Purwanto
Jurnal Biologi Tropis Vol. 21 No. 2 (2021): Mei - Agustus
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i2.2554

Red rice cv. Barak Cenana is a local Indonesian rice which is widely planted in Tabanan Regency, Bali. Barak Cenana red rice has potential as a functional food because it contains a lot of nutrients. However, this rice has an unfavorable character, such as long harvest time so that it can only be harvested once a year, and tall stature that causes plants to lodge easily, reducing its productivity. This study aims to determine the effect of gamma rays on growth and development as well as changes in character to obtain mutants plants that have better character. In this research, Barak Cenana seeds were irradiated using gamma rays with irradiation doses of 100 gy, 200 gy, 400 gy, and 800 gy. Furthermore, radiated seeds were planted in the greenhouse and characterized during the vegetative and generative phases including shoot length, harvest time, chlorophyll content, the number of productive tillers, the number of grain contents, the weight of 1,000 seeds, and the polymorphism profile using Random Amplified Polymorphic DNA Analysis (RAPD). The results of this study indicate that all radiation doses produce plants with random characters. Mutations using gamma rays at doses of 100 gy and 400 gy produced plants with better phenotypic characters than wild-type that is shorter plants, shorter harvest times, and more grain content. In addition, the results of the RAPD analysis confirm that there are genetic changes in irradiated rice. This mutants has the potential to reproduce germplasm and to obtain the next generation of mutants that have higher productivity and shorter plant heights.

Ethyl Methane Sulfonate (EMS) Effect on Mutagenesis in Balinese Red Rice (Oryza sativa cv. Barak Cenana) Ida Bagus Made Arta Dana; Popy Hartatie Hardjo; Maria Goretti Marianti Purwanto; Aloysia Sri Pujiyanti; Indriyani Indriyani
Jurnal Biologi Tropis Vol. 21 No. 3 (2021): September - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i3.2815

Ã¢â¬ÅBarak CenanaÃ¢â¬Â is one of the local red rice (Oryza sativa) with high economic value due to its nutrient content. Some of the agronomic characters such as tall shoot, low number of tiller, and late harvesting are drawbacks to improving production. Ethyl Methyl Sulphonate (EMS) is a chemical mutagen widely used to improve rice characters by mutation breeding. The present research aims to investigate the EMS concentration suitable to induce mutation in Barak Cenana and observe the mutagenesis effect of EMS on some agronomic characters of Barak Cenana. For those purposes, mature seeds of Barak Cenana were treated with various concentrations of EMS (0.25, 0.5, 0.75, 1.0, and 1.1 %) for 24 hours. Seed germination, plant growth, some agronomic character related to productivity were compared to untreated rice. EMS reduced and delayed seed germination and inhibit shoot and root growth in early vegetative stage. The ability of EMS to reduce and delay seed germination and inhibit shoot and root growth were doses dependent. EMS concentration of more than 1.0% was lethal for Barak cenana and EMS concentration at 0.75% was able to reduce rice productivity by increasing seed sterility. EMS concentrations between 0.25 and 0.5% with 24 h of soaking are suitable for inducing mutagenesis in Barak Cenana. Additionally, some mutants with distinct morphology such as sterile plant, semi-dwarf, high tillering capacity, and high chlorophyll contents were produced in this research.

LYCOPENE CONTENT IN SEVERAL AGES OF TOMATO CALLUS (Lycopersiconesculentum Mill. cv. Rampai) Yohanes Alvin Goenawan; Katherine Krisyanti Khodjojo; Popy Hartatie Hardjo; Maria Goretti Marianti Purwanto
Agric Vol. 34 No. 1 (2022)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2022.v34.i1.p89-104

Tomato (Lycopersiconesculentum Mill. cv. Rampai) is rich in lycopene, a carotenoid group compound with many biological activities, one of which is as an antioxidant. Conventional tomato cultivation is not optimal due to the susceptibility of tomato plants to pests and diseases, even though the need for lycopene continues to increase. Plant tissue culture method can be one solution for lycopene production. Callus tomato cv. Rampai was initiated from cotyledons on MS medium (Murashige& Skoog) with the addition of 1 ppm BA (benzyladenine) and 1.5 ppm IAA (indole-3-acetic acid). Callus aged one week to four weeks were harvested and lycopene was extracted by maceration method using hexane:acetone (9:1) as solvent. The presence of lycopene in callus was confirmed by TLC (thin-layer chromatography) (retention factor value/Rf = 0.56) and UV-Vis spectrophotometry (wavelength that gives maximum absorbance = 472 nm). Lycopene content in tomato callus increased with increasing callus age and was directly proportional to the callus growth index. The highest lycopene level was found in callus aged 4 weeks, which was 0.3094 mg/100 g dry weight callus.

LYCOPENE CONTENT IN SEVERAL AGES OF TOMATO CALLUS (Lycopersiconesculentum Mill. cv. Rampai) Yohanes Alvin Goenawan; Katherine Krisyanti Khodjojo; Popy Hartatie Hardjo; Maria Goretti Marianti Purwanto
Agric Vol. 34 No. 1 (2022)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2022.v34.i1.p89-104

Tomato (Lycopersiconesculentum Mill. cv. Rampai) is rich in lycopene, a carotenoid group compound with many biological activities, one of which is as an antioxidant. Conventional tomato cultivation is not optimal due to the susceptibility of tomato plants to pests and diseases, even though the need for lycopene continues to increase. Plant tissue culture method can be one solution for lycopene production. Callus tomato cv. Rampai was initiated from cotyledons on MS medium (Murashige& Skoog) with the addition of 1 ppm BA (benzyladenine) and 1.5 ppm IAA (indole-3-acetic acid). Callus aged one week to four weeks were harvested and lycopene was extracted by maceration method using hexane:acetone (9:1) as solvent. The presence of lycopene in callus was confirmed by TLC (thin-layer chromatography) (retention factor value/Rf = 0.56) and UV-Vis spectrophotometry (wavelength that gives maximum absorbance = 472 nm). Lycopene content in tomato callus increased with increasing callus age and was directly proportional to the callus growth index. The highest lycopene level was found in callus aged 4 weeks, which was 0.3094 mg/100 g dry weight callus.

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