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Hamid Alkhair Badrul
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Environmental Science Materials Science & Nanotechnology Physics

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Fish Analysis Containing Biogenic Amines Using Gas Chromatography Equipped With Flame Ionization And Mass Spectrometer Detectors Muhammad Abdurrahman Munir; Ahlam Inayatullah; Hamid Alkhair Badrul
Science and Technology Indonesia Vol. 6 No. 1 (2021): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1073.894 KB) | DOI: 10.26554/sti.2021.6.1.1-7

Abstract

Biogenic amines generally can be found in fish due to amines in fish undergoing a degradation process. According to the United States Food and Drug Administration (FDA), biogenic amines in fish and fish products can cause harm to consumers if consumed more than 50 µg/mL. Thus, it is important to analyze them. Five biogenic amines such as heptylamine, histamine, tyramine, cadaverine and spermidine were extracted using soaking method with methanol 50% (v/v), afterward they were detected in fish and fish products using gas chromatography – flame ionisation detector (GC-FID) and the biogenic amines structures were confirmed using mass spectrometry (MS). The detection limits (DLs) were range at 1.20 – 2.90 µg/mL. Histamine was detected in fish and fish products such as sardine (Sardinella gibosa) and mackerel (Scomberomorus guttatus) at concentration of 5.96 and 2.69 µg/mL, respectively, whereas cadaverine was found in sardine (Sardinella gibosa) at concentration of 4.96 µg/mL. Histamine concentrations in this study were detected below 50 µg/mL which is below the permissible threshold associated with scombroid poisoing.
Co-Authors Inayatullah, Ahlam Muhammad Abdurrahman Munir