Esther D. Angkouw
Universitas Sam Ratulangi

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STATUS DAN KONDISI PADANG LAMUN DI PERAIRAN PULAU PANIKI DESA KULU KECAMATAN WORI KABUPATEN MINAHASA UTARA Arsida N.A. Kamaludin; Billy Th. Wagey; Calvyn F.A. Sondak; Esther D. Angkouw; Nickson J. Kawung; Khristin I.F. Kondoy
JURNAL PESISIR DAN LAUT TROPIS Vol. 10 No. 3 (2022): JURNAL PESISiR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.10.3.2022.46239

Abstract

Seagrass beds are a very important coastal aquatic ecosystem because they provide great benefits both ecologically in their environment and economically for human life. This study aims to determine the species of seagrass and find out the condition of seagrass beds in the waters of Paniki Island. The method used in this study is the quadratic transect method (perpendicular to the coastline) which is modified from the COREMAP-CTI Seagrass Field Monitoring. The results of the study found 5 species of seagrasses that were identified, namely Thalassia hemprichii, Cymodocea rotundata, Enhalus acoroides, Halodule pinifolia and Halophila ovalis. Thalassia hemprichii has the highest average seagrass closure per species of 23.70% and the lowest average seagrass closure per species which is Halophila ovalis 0.91%. The condition of seagrass beds in the waters of Paniki Island is classified as a category of less rich / unhealthy conditions with a seagrass closing value of 56.24% and is included in the category of dense seagrass cover with a closing value of 51-75%.Keyword: Seagrass Conditions, Cover, Seagrass bed, Paniki Island
Isolation and Antibacterial Activity Test of Seagrass Epiphytic Symbiont Bacteria Thalassia hemprichii from Bahowo Waters, North Sulawesi Ismariani Maarisit; Esther D. Angkouw; Remy E. P. Mangindaan; Natalie D. C. Rumampuk; Henky Manoppo; Elvy Like Ginting
Jurnal Ilmiah PLATAX Vol. 9 No. 1 (2021): ISSUE JANUARY - JUNE 2021
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.9.1.2021.34320

Abstract

Seagrass is a higher plant and has the ability to produce bioactive compounds such as antibacterial. Seagrass is also a host to a variety of bacteria. Bacteria that live in the host will produce the same compounds as the host's body. The utilization of symbiotic bacteria with seagrasses as producers of bioactive compounds such as antibacterial can be used as a solution to reduce excessive seagrass uptake in nature. On the other hand, bacteria have the advantage of being fast and easy to grow and can be mass-produced and more economical. This study aims to isolate and test the antibacterial activity of the epiphytic bacteria of seagrass symbionts. Epiphytic bacteria of seagrass symbionts were grown on Nutrient Agar media directly in the field and bacterial isolation was carried out based on the morphological characteristics of the bacterial isolates. The antibacterial activity test was carried out using the disc method with the test bacteria Stapylococcus aureus, Streptococcus mutans, Escherichia coli, Salmonella thypi, and antibiotics as positive controls. The ability of bacteria to produce antibacterial was indicated by the formation of an inhibition zone around the paper disc containing the epiphytic bacteria of the seagrass symbiont T. hemprichii. A total of 3 isolates of epiphytic bacteria were isolated from T. hemprichii seagrass from Bahowo Waters, Tongkaina Village, Bunaken District, these isolates are namely Epifit 1, Epiphyte 2, and Epiphyte 3. Epiphyte 2 isolate had antibacterial activity against S. mutans, S. aureus, and S. thypi test bacteria, Epiphyte 3 isolate had antibacterial activity against S. mutans, and S. thypi test bacteria.Key words: Bacteria; Antibacterial; T. hemprichii; symbionts; BahowoAbstrakLamun merupakan tumbuhan tingkat tinggi dan memiliki kemampuan menghasilkan senyawa  bioaktif seperti antibakteri. Lamun juga merupakan tempat hidup atau inang dari berbagai bakteri. Bakteri yang hidup pada inang akan menghasilkan senyawa yang sama dengan tubuh inangnya. Pemanfaatan bakteri yang bersimbiosis dengan lamun sebagai produsen senyawa bioaktif seperti antibakteri dapat dijadikan sebagai solusi dalam mengurangi pengambilan lamun yang berlebihan di alam. Dilain pihak, bakteri memiliki keunggulan karena pertumbuhan bakteri yang cepat dan mudah tumbuh, dapat diproduksi secara massal dan lebih ekonomis. Penelitian ini bertujuan untuk mengisolasi dan menguji aktivitas antibakteri dari bakteri epifit simbion lamun T. hemprichii dari Perairan Bahowo.  Bakteri epifit simbion lamun ditumbuhkan pada media NA secara langsung di lapangan dan isolasi bakteri dilaksanakan berdasarkan karateristik morfologi isolat bakteri. Uji aktivitas bakteri dilakukan menggunakan metode cakram dengan bakteri uji S. aureus, S. mutans, E. coli, dan S. thypi dan antibiotik sebagai kontrol positif. Kemampuan bakteri menghasilkan antibakteri ditandai dengan terbentuknya zona hambat disekitar kertas cakram yang mengandung bakkteri epifit simbion lamun T. hemprichii.  Sebanyak 3 isolat bakteri epifit berhasil diisolasi pada lamun T. hemprichii dari Perairan Bahowo, Kelurahan Tongkaina, Kecamatan Bunaken yaitu Epifit 1, Epifit 2, dan Epifit 3. Isolat epifit 3 memiliki aktivitas antibakteri terhadap bakteri uji S. thypi, isolat Epifit 2 terhadap bakteri uji S. mutans, S. aureus, dan S. thypi, isolat Epifit 3 terhadap bakteri uji S. mutans, dan S. thypi.Kata kunci: Bakteri; Antibakteri; T. hemprichii; Simbion; Bahowo