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All Journal Buletin Peternakan Journal of the Indonesian Tropical Animal Agriculture
Rochijan Rochijan
Department Of Animal Production, Faculty Of Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Veterinary Other

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Isolation and identification of bacterial protease enzyme of leather waste Pertiwiningrum, A.; Anggraini, F. D.; Fitrianto, N. A.; Rochijan, R.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 1 (2017): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.1.33-41

Abstract

The objectives of this study were to isolate and identify bacteria which produced protease enzyme from liquid and solid waste of tannery, and their characterization of enzymatic activities. The bacterial isolation used a sample of liquid and solid waste from leather waste which taken from a different waste reservoirs (three liquid waste and one solid waste in unhairing phase). Data of the isolated bacteria, OD 600 nm bacterial growth, the identification of bacteria, and enzyme precipitation with 60% ammonium sulfate were analyzed descriptively. The colony diameter, diameter of clear zone, proteolytic index, and enzymatic activities characterization on difference of pH and temperature were analyzed using Completely Randomized Design, followed by Duncan’s New Multiple Range Test. The second sample from four samples of the isolated bacteria was tested further for their proteolytic activity. The morphology of the colony was circle, white, flat ledges and convex elevation, the basal cell morphology was red, gram-negative, non-motile, catalase positive and gelatin negative. The highest activity of enzyme on pH 11 with activity unit enzyme 45,18±1,77 U/ml and specific enzyme activity 43,19±1,69 U/mg and temperature of 40°C activity unit enzyme 54,02±1,89 U/ml and specific enzyme activity 51,65±1,8 U/mg. The activity of enzyme from protease were precipitated ammonium sulfate 60% showed a higher result of (75,8 U/ml) rather than rough protease.
THE IMPACT OF BALANCED ENERGY AND PROTEIN SUPPLEMENTATION TO MILK PRODUCTION AND QUALITY IN EARLY LACTATING DAIRY COWS Widyobroto, W. P.; Rochijan, R.; Ismaya, I.; Adiarto, A.; Suranindyah, Y. Y.
Journal of the Indonesian Tropical Animal Agriculture Vol 41, No 2 (2016): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.41.2.83-90

Abstract

This research was aimed to determine the impact of balanced energy and protein supplementation with high rumen undegraded protein (HRUP) to milk production and quality in early lactating dairy cows. Twelve early lactating Friesian Holstein cows were divided into two groups (control and HRUP). Both control and HRUP group were fed on a basal diet (forage to concentrate ratio was 60:40; DM basis), with rumen undegraded protein (RUP) levels were 27.47% and 32.78% for control and HRUP, respectively. The experimental diets were given to animals twice daily, morning and afternoon. Water was given by ad libitum. The observed parameters were nutrient intake, quantity and quality of milk production . Data were examined using t-test. Results showed that feed intake, milk production and 4% FCM, milk fat and lactose concentrations, and milk solid non-fat and total solid concentrations were not differed significantly between control and HRUP groups. However, milk protein concentration and production were differed (P
THE PERFORMANCE OF MILK PRODUCTION, TOTAL MILK REVENUE AND REPRODUCTION INDICATORS ON DAIRY SMALLHOLDERS IN YOGYAKARTA AND EAST JAVA, INDONESIA Sudi Nurtini; rochijan rochijan; Budi Guntoro; Budi Prasetyo Widyobroto; Indratiningsih Indratiningsih; Nafiatul Umami
Buletin Peternakan Vol 41, No 2 (2017): BULETIN PETERNAKAN VOL. 41 (2) MEI 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v41i2.23152

Abstract

The purpose of this study was to analyze the performance of milk production, total milk revenue and reproduction indicators of Friesian Holstein Crossbred cows maintained under smallholder’s management system of 122 cows in DIY Province and 345 cows in East Java Province, Indonesia. The study was conducted with survey method and direct observation. A total of 180 Friesian Holstein Crossbred farmers (90 farmers were in DIY and another 90 farmers were from East Java Province) was randomly selected and interviewed used structured questionnaire to assess the milk production, total milk revenue and indicators of reproduction of Friesian Holstein Crossbred cows. The result of the study showed that the average mature equivalent of milk production was 3,810.21±920.10 L/lactation in DIY and 3,717.79±818.44 L/lactation in East Java Province, and the total milk revenue was 12,401,917.87±2.48 IDR per lactation in DIY and 14,647,217.80±3.05 IDR per lactation in East Java Province; day to first mating (postpartum mating), services per conception and days open were 62.34±29.24 days, 2.60±1.32 and 88.58±34.43 days in DIY and 60.62±23.07 days, 2.46±1.26 and 88.67±28.86 days in East Java Province. Based on total milk revenue, the conclusion of this study was Friesian Holstein Crossbred cows maintained under smallholder’s management system in East Java Province better than those in Yogyakarta province (DIY). The mature equivalent of milk production and reproduction indicators of dairy cows (postpartum mating, service per conception and days open) from both provinces showed no significant differences and the value of each parameter reproduction indicators is still within the normal range.
Effect of Dietary Rumen Undegraded Protein (RUP) Level on Nutrient Intake and Digestion of Lactating Dairy Cows Andriyani Astuti; Rochijan Rochijan; Budi Prasetyo Widyobroto
Buletin Peternakan Vol 44, No 4 (2020): BULETIN PETERNAKAN VOL. 44 (4) NOVEMBER 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i4.59155

Abstract

The objective of this study was to examine the effect of different levels of rumen undegraded protein (RUP) in diets of lactating dairy cows on feed intake and nutrient digestion. The experiment was run according to completely randomized design with three treatments of complete feed rations containing different levels of rumen undegraded protein (RUP) and four replications for each treatment. In a digestibility trial, twelve Friesian Holstein cows were divided into three groups at each group consist of four cows and given three dietary treatments containing three levels of RUP, namely 7.43% of total crude protein /CP (P0), 8.49% of total CP (P1), and 9.45% of total CP (P2). Diets consisted of forages (King grass, Pennisetum hybrid) and concentrates at ratio of 50:50 in dry matter basis. The diets contained 18.25% CP and 61.75% total digestible nutrient (TDN). The cows fed diet (P0) were considered the control group. Drinking water was given ad libitum. This experiment was conducted for 21 days of adaptation period and seven days of collection period. In the collection period, samples of feed, refusal feed, and faecal were collected to get the chemical content includes dry matter (DM), organic matter (OM), ether extract (EE), crude fiber (CF), crude protein (CP), nitrogen-free extract (NFE) and total digestible nutrients (TDN). The data obtained were processed with one-way ANOVA (analysis of variance) and the difference existed between the means (P<0.05) would be analyzed with Duncan’s new multiple range test. The results showed that cows fed diet P2 (9.45% RUP in CP) in had significantly higher feed and nutrient intake (DM, OM, CP, CF, EE, TDN) and higher DM and OM digestibility compared to the other two treatments.
Co-Authors A. Adiarto A. Pertiwiningrum Andriyani Astuti Anggraini, F. D. Budi Guntoro Budi Prasetyo Widyobroto Fitrianto, N. A. I. Ismaya Indratiningsih Indratiningsih Nafiatul Umami Sudi Nurtini W. P. Widyobroto, W. P. Y. Y. Suranindyah, Y. Y.