Article Per Year (5 Year)
p-Index From 2019 - 2024
0.778
P-Index
This Author published in this journals
All Journal JURNAL BIOLOGI INDONESIA ANNALES BOGORIENSES Journal of Tropical Crop Science
Sustiprijatno Sustiprijatno, Sustiprijatno
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology

Published : 6 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search
Journal : JURNAL BIOLOGI INDONESIA

 1 
Introduksi Konstruk Gen CsNitr1-L dengan Promotor Ubiquitin melalui Agrobacterium tumefaciens dan Deteksi Molekulernya pada Padi Kultivar Nipponbare Enngarini, Wening; Polosoro, Aqwin; Sustiprijatno, Sustiprijatno; Trijatmiko, Kurniawan Rudi
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i2.3400

Abstract

ABSTRACTNitrogen based fertilizers such as urea and NPK are primary needs for rice farmers. To get significant improvement of crop yield, the more quantity of fertilizers are applied. It make negative impact for surrounding environment. Based on that, the efforts should be done to suppress the demand of fertilizers such as by developing Nitrogen Use Efficiency crops. CsNitr1-L is one of gene that related to Nitrogen Use Efficiency trait in plant. The objectives of this research are to develop the construction of CsNitr1-L gene candidate in pCAMBIA1300-Ubi1 promoter and to obtain the transformants of rice cultivar Nipponbare which contain the construction of CsNitr1-L gene candidate. The construction of pCAMBIA1300::Ubi1::CsNitr1-L has successfully assembled and was transformed to immature embryo of rice cultivar Nipponbare using Agrobacterium tumefaciens strain LBA4404. It was obtained 146 lines of T0 Nipponbare. PCR analysis of T0 Nipponbare lines showed that 66 of them was identified as positive T0 lines contained hptII and CsNitr1-L genes. Transformation efficiency obtained was 11,9%. The result of analysis copy number using Southern Hybridization in positive PCR of T0 lines randomly indicated that 4 lines have a single copy of transgene. Based on these results, it can be concluded that CsNitr1-L gene construct was successfully introduced into the genome of the rice plant cultivar Nipponbare and the positive PCR of T0 lines containing the gene of hptII and CsNitr1-L, also a single copy of the transgene was obtained.Keywords: Nitrogen use efficiency trait, gene construction, rice kultivar Nipponbare
EVALUASI KEMUDAHAN TRANSFER MARKA SSR PADI UNTUK MENGANALISIS KERAGAMAN GENETIK FAMILI POACEAE TOLERAN KEKERINGAN Fatimah, Fatimah; Masumah, Masumah; Prasetiyono, Joko; Sustiprijatno, Sustiprijatno
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3764

Abstract

ABSTRACTAnticipating climate change and its impacts on crop production, it requires the development of adaptive rice varieties that respond to drought stress and minimize the damage while keeps growing and maintaining yield. The aim of this study was to evaluate the transferability of rice simple sequence repeat (SSR) markers to Poaceae family and analyze the genetic diversity among 16 accessions of Poaceae family (12 genera and 14 species) using 41 rice SSR markers. The result revealed that the transferability of rice SSR markers was varied. High amplification produced in rice group (>70%) and low amplification in grass group (<30%) with an average of 35.2%. A total of 128 cross-species alleles were identified with an average of 3 alleles/locus. The value of gene diversity ranged from 0.15 to 0.83 with an average of 0.53 and the value of Polymorphic Information Content (PIC) ranged from 0.14 to 0.80 with an average of 0.46. The results of phylogenetic analysis determined two clusters at similarity coefficient of 0.72. The first cluster consisted of 14 accessions from Poaceae family (12 genera and 14 species) while the second cluster consisted of two cultivated rice varieties (Inpari 30 and Situ Bagendit). The genetic relatedness data revealed from this study could be used as basic information for parental selection. The 14 accessions of Poaceae family have a potential for drought tolerant donor and separated clearly from cultivated rice varieties (Inpari 30 and Situ Bagendit) for recipient parents in spike-stalk injection method (SIM) to develop drought tolerant rice varieties.  Keywords: Cross amplification, Drought tolerant, Inpari 30, Situ Bagendit   
EVALUASI KEMUDAHAN TRANSFER MARKA SSR PADI UNTUK MENGANALISIS KERAGAMAN GENETIK FAMILI POACEAE TOLERAN KEKERINGAN Fatimah, Fatimah; Masumah, Masumah; Prasetiyono, Joko; Sustiprijatno, Sustiprijatno
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3764

Abstract

ABSTRACTAnticipating climate change and its impacts on crop production, it requires the development of adaptive rice varieties that respond to drought stress and minimize the damage while keeps growing and maintaining yield. The aim of this study was to evaluate the transferability of rice simple sequence repeat (SSR) markers to Poaceae family and analyze the genetic diversity among 16 accessions of Poaceae family (12 genera and 14 species) using 41 rice SSR markers. The result revealed that the transferability of rice SSR markers was varied. High amplification produced in rice group (>70%) and low amplification in grass group (<30%) with an average of 35.2%. A total of 128 cross-species alleles were identified with an average of 3 alleles/locus. The value of gene diversity ranged from 0.15 to 0.83 with an average of 0.53 and the value of Polymorphic Information Content (PIC) ranged from 0.14 to 0.80 with an average of 0.46. The results of phylogenetic analysis determined two clusters at similarity coefficient of 0.72. The first cluster consisted of 14 accessions from Poaceae family (12 genera and 14 species) while the second cluster consisted of two cultivated rice varieties (Inpari 30 and Situ Bagendit). The genetic relatedness data revealed from this study could be used as basic information for parental selection. The 14 accessions of Poaceae family have a potential for drought tolerant donor and separated clearly from cultivated rice varieties (Inpari 30 and Situ Bagendit) for recipient parents in spike-stalk injection method (SIM) to develop drought tolerant rice varieties.  Keywords: Cross amplification, Drought tolerant, Inpari 30, Situ Bagendit   
INTRODUKSI KONSTRUK GEN CSNITR1-L DENGAN PROMOTOR UBIQUITIN MELALUI AGROBACTERIUM TUMEFACIENS DAN DETEKSI MOLEKULERNYA PADA PADI KULTIVAR NIPPONBARE Enngarini, Wening; Polosoro, Aqwin; Sustiprijatno, Sustiprijatno; Trijatmiko, Kurniawan Rudi
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i2.3400

Abstract

ABSTRACTNitrogen based fertilizers such as urea and NPK are primary needs for rice farmers. To get significant improvement of crop yield, the more quantity of fertilizers are applied. It make negative impact for surrounding environment. Based on that, the efforts should be done to suppress the demand of fertilizers such as by developing Nitrogen Use Efficiency crops. CsNitr1-L is one of gene that related to Nitrogen Use Efficiency trait in plant. The objectives of this research are to develop the construction of CsNitr1-L gene candidate in pCAMBIA1300-Ubi1 promoter and to obtain the transformants of rice cultivar Nipponbare which contain the construction of CsNitr1-L gene candidate. The construction of pCAMBIA1300::Ubi1::CsNitr1-L has successfully assembled and was transformed to immature embryo of rice cultivar Nipponbare using Agrobacterium tumefaciens strain LBA4404. It was obtained 146 lines of T0 Nipponbare. PCR analysis of T0 Nipponbare lines showed that 66 of them was identified as positive T0 lines contained hptII and CsNitr1-L genes. Transformation efficiency obtained was 11,9%. The result of analysis copy number using Southern Hybridization in positive PCR of T0 lines randomly indicated that 4 lines have a single copy of transgene. Based on these results, it can be concluded that CsNitr1-L gene construct was successfully introduced into the genome of the rice plant cultivar Nipponbare and the positive PCR of T0 lines containing the gene of hptII and CsNitr1-L, also a single copy of the transgene was obtained.Keywords: Nitrogen use efficiency trait, gene construction, rice kultivar Nipponbare
Co-Authors Enngarini, Wening Enngarini, Wening fatimah Fatimah Iskandar, Hayati Minarsih Joko Prasetiyono Kurniawan Rudi Trijatmiko, Kurniawan Rudi Masumah, Masumah Mose, Windi Pambudi, Jembar Polosoro, Aqwin PUTRANTO, Riza Arief Suhandono, Sonny TATI KRISTIANTI