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Vidia Royanti
Universitas Lampung
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ISOLASI DAN KARAKTERISASI BACILLUS LIPOLITIK DARI TANAH KEBUN RAYA LIWA Vidia Royanti; Kusuma Handayani; Chistina Nugroho Ekowati; Sumardi Sumardi
Gunung Djati Conference Series Vol. 18 (2023): Prosiding Seminar Nasional Biologi (SEMABIO) Tahun 2022
Publisher : UIN Sunan Gunung Djati Bandung

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Abstract

Bacillus is a bacterium that has the ability to survive in unfavorable environmental conditions, one of which is in nutrient-poor soils such as the Liwa Botanical Gardens. Bacillus isolates have been obtained from the soil of the Liwa Botanical Gardens which have lipolytic abilities. This study aimed to determine the activity of lipolytic bacteria isolates from Bacillus from Liwa Botanical Gardens. In this study, Bacillus was isolated from the soil of the Liwa Botanical Gardens using a graded dilution method, using Nutrient Agar media. The Bacillus isolates obtained were then purified in Nutrient agar media. Bacillus isolates were inoculated in lipase test media with a composition of 0.01% methyl red, 10 g peptone, and Nutrient Agar, 5% sterile olive oil, 2.5% Tween-80, 5 g NaCl, 0.1 g CaCl2.2H2O. The results of this study obtained 5 isolates of Bacillus which had fairly good growth, namely isolates BP 13, BP 14, BP16, TMA 13, TSR 5. With the results obtained the largest lipolytic activity was isolate BP 16 with an average index value of 6.34 cm while the lowest lipolytic activity was TMA13 isolate with an average index value of 2,345 cm.
INDEKS KEANEKARAGAMAN BAKTERI BACILLUS SP. DARI TANAH KEBUN RAYA LIWA Kusuma Handayani; Vidia Royanti; Chistina Nugroho Ekowati
Gunung Djati Conference Series Vol. 18 (2023): Prosiding Seminar Nasional Biologi (SEMABIO) Tahun 2022
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1277.682 KB)

Abstract

Bacillus sp is a bacterium that is commonly found in soil, water, air and plant debris, has the ability to produce enzymes and degrade natural substrates so that it contributes to the nutrient cycle. The Kebun Raya Liwa area is located at an altitude between 890 to 948 meters above sea level where at this altitude is included in a hilly area. With varying topographic conditions so that it has different soil conditions. This can trigger the diversity of Bacillus sp. The purpose of this study was to determine the diversity index of Bacillus sp in the Liwa Botanical Gardens soil. This research was conducted at the microbiology laboratory of the Mathematics and Natural Sciences faculty, Lampung University. The method used is sampling using five different types of soil, namely litter soil, biopori soil, arase sloping soil, ordinary soil, and ordinary arase soil. The isolation technique uses the pour plate method with multilevel dilutions. Calculation of diversity using a calculation method that refers to the calculation of Shanon Wiener. The results of this study obtained the highest diversity of Bacillus sp in litter soil with a total index of 2.488464 indicating a moderate level of diversity while the lowest total value was obtained in biopore soil with a value of 1.351604 indicating a moderate level of diversity. The high and low diversity of Bacillus sp bacteria can be caused by environmental conditions. In this study, the dominant morphology of Bacillus sp is circular colony shape.
Co-Authors Chistina Nugroho Ekowati Kusuma Handayani Sumardi, Sumardi