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All Journal Indonesian Chimica Letters
NG. Krishnabudi
Department of Economics, Faculty of Economics and Business, University of Jember
Chemistry

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Determination Kinetic Parameters of Endo-β-1,4-D-Xylanase from Abdomenal Termites with Xylan Oat and Birchwood Anak Agung Istri Ratnadewi; Linda Faiqotul Himmah; Tri Mulyono; Wuryanti Handayani; NG. Krishnabudi; Sudarko
Indonesian Chimica Letters Vol. 1 No. 1 (2022)
Publisher : Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (564.421 KB) | DOI: 10.19184/icl.v1i1.3

Abstract

Parameters kinetic (KM, VMax, and kCat) endo-β-1,4-D-xylanase under optimum conditions with oat spelt xylan and birchwood substrate have been investigated in this study. Hydrolysis of endo-β-1,4-D-xylanase using variation of substrate concentration (b/v) ranging from 0.2 to 1.2%. Variation of incubation time is up to 20 hours with 4 hours interval at the optimum temperature of the enzyme, 40°C. The results obtained from this study were the KM of endo-β-1,4-D-xylanase for oat spelt xylan and birchwood were 4.10 mg/ml and 0.681 mg/ml, respectively. VMax values, and kCat for oat spelt xylan substrate of 0.28 mg/ml.jam and 1.7 x 10-3 s-1. While VMax, and kCat for birchwood substrate that is 0.117 mg/U/jam and 7 x 10-4 s-1. From the results of this study we found that endo-β-1,4-D-xylanase can hydrolaze substrates which have differences solubility.
Co-Authors Anak Agung Istri Ratnadewi Linda Faiqotul Himmah S. Sudarko Tri Mulyono Wuryanti Handayani