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All Journal FLUIDA Industrial Research Workshop and National Seminar
Rintis Manfaati
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Humanities Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Education Electrical & Electronics Engineering Energy Engineering Environmental Science Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Other

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Hydrolysis of Waste Cooking Oil Using Rhizopus oryzae to Produce Free Fatty Acids Rintis Manfaati; Prans Connery Manurung; Muhamad Nur Rojab; Keryanti Keryanti
Fluida Vol 16 No 1 (2023): FLUIDA
Publisher : Jurusan Teknik Kimia, Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/fluida.v16i1.4496

Abstract

Waste cooking oil is waste produced from palm oil after it has been heated and fried at high temperatures, and it can pollute the environment. One effort to reuse waste cooking oil is a fermentation processe that produces free fatty acids with the help of Rhizopus oryzae as a biocatalyst. Variations in initial substrate concentration ranged from 10 g/L to 70 g/L, followed by varied types of nitrogen sources, namely malt extract, beef extract, (NH2)2CO, NH4Cl, and (NH4)2SO4 at a concentration of 70 g/L to determine free fatty acid concentration. Fermentation was carried out for 7 days. The analysis carried out included the concentration of free fatty acids, biomass, and YP/X value. The optimum initial used cooking oil substrate was obtained at a concentration of 30 g/L with a YP/X value of 13.63%, a free fatty acid concentration of 2.13 g/L and a dry cell weight of 15.48 g/L. The best nitrogen source is beef extract with a Yp/x value of 11.78%, a free fatty acid concentration of 2.02 g/L and a dry cell weight of 17.0 g/L
Pengaruh Konsentrasi Tepung Kulit Nanas Pada Fermentasi Dengan Metode SHF dan SSF Untuk Menghasilkan Etanol Keryanti Keryanti; Hanafiah Herliana; Nani Anggraeni; Rintis Manfaati
Prosiding Industrial Research Workshop and National Seminar Vol 14 No 1 (2023): Vol 14 (2023): Prosiding 14th Industrial Research Workshop and National Seminar
Publisher : Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/irwns.v14i1.5443

Abstract

Limbah kulit nanas masih memiliki kandungan pati 17.53% (b/b), protein kasar 8.78% (b/b), lemak kasar 1.15% (b/b) dan gula reduksi 13.65% (b/b) sehingga dapat menjadi alternatif bahan baku fermentasi untuk menghasilkan etanol. Produksi etanol dari bahan pati dan selulosa seperti tepung kulit nanas beberapa kali telah dilakukan, namun etanol yang dihasilkan kurang optimal sehingga membutuhkan alternatif proses yang dapat meningkatkan kualitas etanol. Upaya peningkatan produksi etanol dari tepung kulit nanas dapat dilakukan dengan menggunakan dua metode yaitu Separated Hydrolysis and Fermentation (SHF) dan Simultaneous Saccharification and Fermentation (SSF). Tujuan penelitian ini untuk mengetahui pengaruh variasi konsentrasi tepung kulit nanas terhadap kadar etanol hasil fermentasi dengan metode SHF dan SSF. Variasi konsentrasi tepung kulit nanas yang digunakan (g/L) yaitu 20, 40, 60 dan 80. Proses fermentasi dilakukan pada reaktor 250 mL selama 96 jam dengan penambahan inokulum Saccharomyces cerevisiae sebanyak 20% (v/v). Pada penelitian ini digunakan enzim selulase sebesar 5% (v/v) dari bahan baku yang telah dilarutkan untuk proses hidrolisis pada metode SSF serta katalis HCl 0.1 N (b/v) pada metode SHF. Pengujian dari hasil penelitian ini analisis kadar glukosa dengan metode DNS dan analisis kadar etanol menggunakan refraktometer serta HPLC. Dari hasil penelitian ini diperoleh kadar etanol tertinggi pada konsentrasi tepung kulit nanas 80 g/L sebesar 7.99% untuk metode SHF dan 13.01% untuk metode SSF.
Co-Authors Hanafiah Herliana Keryanti, Keryanti Muhamad Nur Rojab Nani Anggraeni Prans Connery Manurung