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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Edi Erwan
Faculty of Agriculture and Animal Science, State Islamic University of Sultan Syarif Kasim Riau, H.R. Soebrantas KM. 15 Street, Pekanbaru, 28293, Riau
Agriculture, Biological Sciences & Forestry Environmental Science

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The Effectiveness of Adding Different Sucrose in Tris-Egg Yolk Diluent on the Sperm Fertility of Sexing Swamp Buffalo (Bubalus bubalis): Adding Different Sucrose in Tris-Egg Yolk Diluent on the Sperm Fertility of Sexing Swamp Buffalo Yendraliza Yendraliza; Allif Bukhori; Edi Erwan; Ria Sari Gail Sianturi; Diana Andrianita Kusumaningrum; Muhamad Rodiallah; Zumarni
Journal of Tropical Life Science Vol. 13 No. 2 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.02.17

Abstract

Spermatozoa sexing requires a diluent that can protect and provide an optimal environment to maintain the quality of spermatozoa. This study targets to determine the effectiveness of adding sucrose to tris-egg yolk diluent on the sperm quality of the swamp buffaloes produced by sexing with the egg albumin method. Semen was collected once a week for 12 consecutive weeks with an artificial vagina from three male buffaloes at the Indonesian Center for Animal Research and Development (ICARD) in April - June 2021. Semen was separated by sedimentation using 10% and 30% egg albumins. Semen from sexing was divided into four diluent treatments, namely 20% tris-egg yolk (TEY), TEY+0% sucrose, TEY+0.2% sucrose, TEY+0.3% sucrose, and TEY+0.4% sucrose. Parameters measured included: motility, viability, intact plasm membrane, and intact acrosome cap of buffalo sperm. The results showed the motility of X sperm: 43.75-47.5%, Y sperm: 40-45%, the viability of X sperm: 78.25-79.75% and Y sperm: 77.25-79.75%, intact plasm membrane of X sperm: 70.5-71.5% and Y sperm: 70.5-71%, and viable acrosome-intact for X sperm: 79-80% and Y sperm: 78.75-79.5%. The addition of sucrose up to 0.4% into tris-egg yolk diluent was proven can maintain motility, viability, and integrity of cell membrane and acrosome of spermatozoa of water buffalo that was sexed with egg albumin.
Co-Authors Allif Bukhori Diana Andrianita Kusumaningrum Muhamad Rodiallah Ria Sari Gail Sianturi Yendraliza Yendraliza Zumarni