Irisa Trianti
Departemen Hama Dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Brawijaya

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Baculovirus Surface Display Using Infuenza Neuraminidase (NA) Transmembrane Anchor Trianti, Irisa; Akeprathumchai, Saengchai; Mekvichitsaeng, Phenjun; Poomputsa, Kanokwan
Journal of Tropical Life Science Vol 6, No 3 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.03.12

Abstract

Baculovirus surface display has been employed as an excellent tools for presentation of foreign peptides and proteins on virus surface with native conformation, functions and immunogenicity. A baculovirus major envelope protein, gp64, or a capsid protein, vp39 are generally used as fusion partners for displaying of polypeptides on the surface of virions. Alternatively, a membrane anchoring domain of vesicular stomatitis virus G protein (VSV-G) can also be used. In this study, an influenza neuraminidase (NA) was proposed as a new membrane anchor for the display of Angiotensin II (AngII), DRVYIHPFHL, peptides. The AngII peptides were inserted into NA by replacing NA amino acid number 60-67 with AngII, and then integrated into a baculovirus genome. A recombinant baculovirus expressing the NA fusion-AngII peptides was generated from infected insect cells. Those peptides were found to express and translocated on the membrane of the baculovirus infected insect cell (Sf9 cell) as detected by immunocytochemistry using anti-AngII monoclonal antibody. Upon budding of the recombinant baculovirus progenies through the insect cells membrane, the recombinant NA-AngII peptides was acquired to envelopes of the new baculovirus progenies. The conformation of NA on baculovirus surface was not affected by the deletion, as the 55 kDa band of NA can be detected from Western Blotting analysis by specific anti-NA monoclonal antibody. In addition, the same protein was also found by anti-AngII antibody indicating that the AngII peptides had been successfully fused with the recombinant NA. Interestingly, electron microscopy analysis demonstrated that not only the recombinant baculovirus displaying AngII peptides were generated by infected insect cells, but also the NA virus-like-particle displaying AngII peptides.
EKSPLORASI KHAMIR SIMBION PADA SALURAN PENCERNAAN LARVA Oryctes rhinoceros L.: EXPLORATION OF SYMBIOTIC YEAST FROM THE DIGESTIVE TRACT OF Oryctes rhinoceros L. LARVAE Irisa Trianti; Yogo Setiawan; Tomo Agus Supriyantono; Sholikah Widyanitta Rachmawati
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 11 No. 2 (2023)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jurnalhpt.2023.011.2.5

Abstract

Oryctes rhinoceros merupakan serangga fitofag dan telah dilaporkan sebagai hama di beberapa negara. Larva O. rinocheros makan pada bahan organik (bagian tanaman yang membusuk) dan tidak menyebabkan kerusakan ekonomi. Seperti pada kebanyakan serangga, O. rhinoceros berinteraksi dengan mikroorganisme pada sistem pencernaanya. Selain itu, mikroorganisme dalam saluran pencernaan serangga juga merupakan sumber daya alam yang bermanfaat untuk kepentingan manusia, seperti enzim-enzim yang memiliki arti penting dalam bidang industri dan digunakan sebagai agens bioremediator untuk mengatasi masalah pencemaran lingkungan. Penelitian ini bertujuan untuk mengeksplorasi dan mempelajari keanekaragaman khamir dalam saluran pencernaan larva O. rhinoceros. Pada studi ini, khamir diisolasi dari saluran pencernaan instar tiga O. rhinoceros dan ditumbuhkan pada media Yeast Malt Agar (YMA). Berdasarkan ciri makroskopis dan mikroskopis, 7 isolat khamir yang diperoleh teridentifikasi sebagai Candida sp. (isolat K1 dan K4), Pichia sp. (isolat K3), dan Debaromyces sp.(K2, K5, K6 dan K7).
Efficacy of Biofungicide with Active Ingredients Trichoderma sp. Against Late Blight Disease (Phytophthora infestans) in Potato Plants Abdul Latief Abadi; Irisa Trianti; Fery Abdul Choliq; Antok Wahyu Sektiono; Novi Dwi Yulianti
Agro Bali : Agricultural Journal Vol 7, No 1 (2024)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v7i1.1591

Abstract

The cultivation of potatoes in Indonesia faces challenges, particularly a decline in production attributed to the devastating impact of late blight caused by the fungus P. infestans. Traditional control methods involve the frequent use of chemical pesticides, which pose environmental and health risks. To address this, the research aimed to assess the efficacy of biofungicide containing Trichoderma sp. as a biological agent in controlling late blight on potatoes. The study, conducted in Sumberbrantas Village, Bumiaji District, Batu City, East Java, and the plant disease laboratory at Brawijaya University from January to April 2023, employed both in vivo and in vitro experiments. A Randomized Block Design (RBD) was used for in vivo research, while a Completely Randomized Design (CRD) was employed for in vitro studies, each comprising five concentration treatments with five repetitions. Parameters such as disease intensity, efficacy level, potato production, and biofungicide inhibition were observed. Analysis of variance and the Tukey Honestly Significant Difference (HSD) test were applied to the data and processed using Microsoft Excel. The results indicated that biofungicide with Trichoderma sp. concentrations of 6 ml/l, 4.5 ml/l, 3 ml/l, and 1.5 ml/l effectively reduced the intensity of late blight on potatoes, with efficacy levels exceeding 50%. The highest potato production occurred with a concentration of 6 ml/l, yielding 15.44 tons/ha, followed by 14.67 tons/ha for the 4.5 ml/l concentration. The biofungicide with a concentration of 6 ml/l exhibited the lowest disease intensity at 14.28% and the highest efficacy at 83.08%. Inhibition tests demonstrated that concentrations of 6 ml/l and 4.5 ml were highly effective, inhibiting P. infestans growth by an average of 62.04% and 59.90%, respectively. These findings highlight the potential of biofungicide with Trichoderma sp. in managing late blight on potatoes, providing a sustainable and environmentally friendly alternative to chemical pesticides.