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All Journal Seminar Nasional Teknik Kimia Kejuangan
Adel Frisca Rahayu
Program Studi Teknologi Rekayasa Kimia Industri, Fakultas Vokasi, Institut Teknologi Sepuluh Nopember Jl. Raya ITS Sukolilo Surabaya 60111, Telp. (031) 5937968 Fax (031) 5965183
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Engineering Environmental Science

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Pengaruh Variasi Bahan Pengendap pada Proses Purifikasi Enzim Bromelin dari Bonggol Nanas (Ananas Comosus L.) Warlinda Eka Triastuti; Sunia Rahma Cahyaning Tyas; Hanifah Fauziyah Zahrah; Kabira Bennani; Erika Desi Cahyani; Tatya Annur Ramadita; Adel Frisca Rahayu
Prosiding Seminar Nasional Teknik Kimia "Kejuangan" 2023: PROSIDING SNTKK 2023
Publisher : Seminar Nasional Teknik Kimia "Kejuangan"

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Abstract

Waste from processed pineapples (pineapple core) is currently underutilized, despite the high content of the bromelain enzyme found in the fruit. This research aims to determine the effect of precipitating agents on the purification process of bromelain enzyme production using a phosphate buffer on the resulting enzyme yield and to analyze the effect of the precipitating agents on the purification process of bromelain enzyme production using a phosphate buffer on the bromelain enzyme activity. The variables used in this study were a 0.1 M phosphate buffer, 55%; 60%; 65%; 70%; 75% aseton concentration, 5%; 10%; 15%; 20%; 25% NaCl concentration, and 60%; 65%; 70%; 75%; 80% ethanol concentration. This study will be conducted in three stages, namely Extraction, Purification, and Drying. The highest yield was obtained with 60% aseton concentration (1.44%), 65% ethanol concentration (1.42%), and 15% NaCl concentration (2.2%). The optimal enzyme activity was obtained with 60% aseton concentration (20225.616 U/gr), 65% ethanol concentration (6266.765 U/gr), and 15% NaCl concentration (2281.357 U/gr). In conclusion, the best result for producing bromelain enzyme powder from pineapple cores is by using a 60% aseton concentration solvent.
Co-Authors Erika Desi Cahyani Hanifah Fauziyah Zahrah Kabira Bennani Sunia Rahma Cahyaning Tyas Tatya Annur Ramadita Triastuti, Warlinda Eka