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All Journal Jurnal Veteriner Nusantara
Nemay A. Ndaong
Universitas Nusa Cendana
Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Public Health Veterinary

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Uji potensi ekstrak etanol daun gewang (Corypha utan lamk) sebagai antihelmintik terhadap cacing Ascaris suum secara in vitro Trianto Umbu Reku; Nemay A. Ndaong; Julianty Almet
Jurnal Veteriner Nusantara Vol 2 No 1 (2019)
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v2i1.1089

Abstract

The system of pig farm in East Nusa Tenggara (NTT) province is a traditional farm so that pigs are susceptible to diseases caused by endoparasite. Parasite species commonly invested in pigs is Ascaris suum. Treatment used and believed to have an effect as anti-endoparasite for infected pigs is leaves of Gewang (Corypha utan Lamk). The aims of this study were to assess the potency of ethanol leaves extract of gewang against worm Ascaris suum in vitro and to know the potential compound in the leaves by phytochemical test. Samples of 60 Ascaris suum were collected from small intestines of pigs. Extract was obtained by evaporation and maceration methods, and then divided into several concentrations, which were 5%, 10% and 15%. Control group used physiological saline 0.9%. The results showed that the 15% extract had the fastest time (21.33 hours) to kill the parasite, while the 10% and 5% extract needed 28.33 and 42.33 hours respectively. Based on phytochemical test, the extract contained saponins and tannins. The conclusion of this study is that the extract of ethanol leaves of Gewang has good potential as anthelmintic for Ascaris suum.
Uji potensi ekstrak etanol daun nimba (Azadiractha indica) sebagai bahan pengawet pada ikan tongkol (Auxis thazard) Ade Lestrari Rambu Leba; Nemay A. Ndaong; Maria AEGA Gelolodo
Jurnal Veteriner Nusantara Vol 2 No 1 (2019)
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v2i1.1093

Abstract

This study aimed to the capability of the ethanol leaf extract obtained from neem (Azadiractha indica) as preservatives for frigate mackerel (Auxis thazard). Analysis was carried out by looking at the storability of the fish with two parameters, which are pH and total plate count (TPC). The samples were treated with different treatments by using different extract concentrations. This study was an experimental laboratory research using completely randomized design method with three replications of factorial 5x4. There were 5 treatments, 10% formalin (positive control), ice (negative control), 5% concentration of ethanol extract of neem leaves (K1), 10% concentration of ethanol extract of neem leaves (K2), 15% concentration of ethanol extract of neem leaves (K3). The mackerel were soaked for two hours, then stored at room temperature. The measurement of pH and TPC were done at 0 hour, 2 hours, 7 hours, and 12 hours. The result of this study showed that pH and TPC values gradually increased along with the length of time of observation. The ethanol leaf extract of neem (Azadiractha indica) as preservatives on the mackerel had storability for 2-7 hours. It can be concluded that the higher concentration of ethanol leaf extract of neem, the higher inhibitory effect on bacterial growth will be.
Persembuhan luka incisi kulit mencit (Mus musculus) dengan pemberian ekstrak etanol teripang getah (Holothuria leucospilota) Srivikaya R.W. Ollu; Putri Pandarangga; Nemay A. Ndaong
Jurnal Veteriner Nusantara Vol 2 No 1 (2019)
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v2i1.1096

Abstract

The purpose of this research is to compare the effect of the ethanol black sea cucumber (Holothuria leucospilota) extract with betadine in the healing wound process of incised skin of mice (Mus musculus) through observation of gross pathology and histopathology. This research used mice with age of 6-8 weeks, male and divided into three groups, which were negative control (aquades), the positive control (betadine), and the treatment (ethanol black sea cucumber extract). All mice were incised by surgical blade in the middle of the back with the depth of up to layer of dermis. Furthermore, the wounds were lubricated directly by aquades, betadine and ethanol black sea cucumber extract. The gross pathology data were obtained in eight days through the observation of color, dry cuts, the closing time, the scab formation and the increase the length of hair around and the wound size. The parameters used in histopathology analysis of healing wounds process were the presence of increasing number of cells fibroblast, angiogenesis, and the number of the inflammation cells around the wound. The results showed that the effect of ethanol black sea cucumber (H. leucospilota) extract accelerated the wound healing process in the incised skin of mice (Mus musculus). It was caused by the bioactive content of black sea cucumber, such as saponin, flavonoid, lectin and amino acids which can speed up wound healing wound.
Co-Authors Ade Lestrari Rambu Leba Julianty Almet Maria Aega Gelolodo Srivikaya R.W. Ollu Trianto Umbu Reku