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Alfi Nurwahidah
Universitas Billfath
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Mathematics Physics

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POTENSI AIR REBUSAN KEDELAI SEBAGAI MEDIA ALTERNATIF PERBANYAKAN BAKTERI Pseudomonas fluorescens SEBAGAI AGEN HAYATI Alfi Nurwahidah; Trisnani Alif
Jurnal Matematika dan Sains (JMS) Vol. 2 No. 1 (2022): Jurnal Matematika dan Sains (JMS)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.552273/jms.v2i1.127

Abstract

Pseudomonas fluorescens is a bacterial antagonist that is widely used as a biological control agent. Propagation of Pseudomonas fluorescens is mostly done with synthetic agar media. This requires an expensive cost, therefore there is a need for alternative media for bacterial propagation. This study aims to examine the growth of Pseudomonas fluorescens in liquid media of soybean cooking water. The research was carried out at the Office of the TPH Protection Coordinator for the Bojonegoro Region. The research method used is descriptive quantitative method. carried out on the number of bacterial colonies. The results of this study indicate that soybean cooking water can be used as a medium for propagation of Pseudomonas fluorescens bacteria and has a high bacterial density with the number of bacterial colonies of 6.5×108 CFU/ml in the first week.
Pengaruh Aplikasi Beauveria Bassiana Terhadap Intensitas Serangan dan Mortalitas Larva Spodoptera frugiperda Pada Tanaman Jagung (Zea mays L.). Alfi Nurwahidah; Trisnan Alif
Jurnal Matematika dan Sains (JMS) Vol. 3 No. 1 (2023): Jurnal Matematika dan Sains (JMS)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55273/jms.v3i1.189

Abstract

S. frugiperda is a pest that attacks corn plants by eating young shoots/leaves. Leaves that are attacked by S. frugiperda will have many hoisting holes from the edges to the inside. If the attack of S. frugiperda is not immediately controlled, the losses will be crop failure. This study aims to determine the effectiveness of the use of several concentrations of B. bassiana on the intensity of pest attack and mortality of S. frugiperda larvae on maize. This research was conducted in the green house laboratory of Billfath University using a Completely Randomized Design (CRD) consisting of five treatments and four replications. The concentrations of B. bassiana used included K1= negative control (without application), K2= B. bassiana 5 ml/L, K3= B. bassiana 15 ml/L, K4= B. bassiana 25 ml/L and K5= positive control (Chemical insecticide Tiamethoxam 25%) 0.01 g/L. The results showed that the application of the fungus B. bassiana with a concentration of 25 ml/L was effective in controlling S. frugiperda pests on maize (Zea mays L.) which resulted in an attack intensity of 12.50% and larval mortality of 95%.. Keywords: entomopathogenic fungi, B. bassiana, S. frugiperda, corn
Co-Authors Trisnan Alif Trisnani Alif