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All Journal Jurnal Bahana Kesehatan Masyarakat (Bahana of Journal Public Health) Journal Health Applied Science And Technology (JHAST)
Dewi Hartati
IKesT Muhammadiyah Palembang
Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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PERBEDAAN HASIL PEMERIKSAAN RETIKULOSIT SEBELUM DAN SESUDAH AKTIVITAS FISIK Dewi Hartati; Indah Sari; Zahra Tulhaya Jannah
jurnal kesehatan terapan sains dan teknologi Vol 1 No 1 (2023): Journal Health Applied Science And Technology (JHAST)
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/jhast.v1i1.2

Abstract

Introduction: Reticulocytes are young blood cells that have just been released into the bloodstream from the bone marrow. Reticulocytes have characteristics that still have ribosomal RNA remnants. Physical activity is a body movement produced by skeletal muscles and requires energy, including activities performed while working, playing, doing household chores, exercising, and traveling.. Purpose: The purpose of this study was to determine the differences in the results of reticulocyte examination before and after physical activity. Research Method: This research was conducted in 2 laboratories, blood sampling at the Hematology Laboratory, Faculty of Science and Technology, Muhammadiyah Palembang Institute of Health Sciences and Technology, and at the Palembang Health Laboratory Center. The number of samples used was 17 with 2 treatments using purposive sampling technique. Based on the results of reticulocyte examination before and after physical activity using the sysmex-XN1000 hematology analyzer. Results: The results showed that before physical activity produced an average of 1.24% while those after physical activity produced an average of 1.12%. In this study, there was a decrease that was not far from its normal value. Analysis of the data using the Wilcoxon test results obtained sig 0.209 where p≥0.05. Conclusion: The conclusion of this study is that there is no difference between before and after physical activity.
PERBANDINGAN KADAR ENZIM ALKALINE PHOSPHATASE (ALP) PADA SERUM HEMOLISIS RINGAN DAN NON HEMOLISIS Tria Agustina; Bastian Bastian; Dewi Hartati
Jurnal Bahana Kesehatan Masyarakat (Bahana of Journal Public Health) Vol 7 No 1 (2023): Jurnal Bahana Kesehatan Masyarakat (Bahana of Journal Public Health)
Publisher : Poltekkes Kemenkes Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35910/jbkm.v7i1.619

Abstract

Background: Alkaline Phosphatase (ALP) is a hydrolase enzyme that is first produced by the liver epithelium and osteoblasts (cells that form new bone), this enzyme is mostly found in the liver and bones. ALP enzyme examination can be affected if there is damage to the cells, causing haemolysis. Haemolysis can be affected by phlebotomy techniques at an inappropriate pre-analytical stage, so that haemolysis can cause increased concentrations in red blood cells compared to serum or plasma, thus giving false concentration results. This study aims to determine the differences in ALP enzyme levels in mild haemolysed serum and non-haemolysed serum. Methods: The study population included all 17 male students of DIV Medical Technology Laboratory, Faculty of Science and Technology Muhammadiyah Palembang, by differentiating inclusion, namely aged 17 to 22 years, male sex and willing to be respondents. Samples in the form of mild hemolysis and non-hemolysis serum were taken from the respondents. The stages of the research began with taking venous blood samples, processing the blood into non-hemolytic serum and mild hemolytic serum, and ALP examination using the Biosystem BA 200 tool. Results: This study showed that the difference between ALP enzyme levels in non-haemolytic and haemolytic samples was 73.9 U/L or 55%. The data analysis test used the Paired T test which obtained p ≥ 0,000. Conclusion: There are differences in results between mild haemolysed serum and non-haemolysed serum.
Co-Authors Bastian Bastian Indah Sari Tria Agustina Zahra Tulhaya Jannah