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PERBANDINGAN PEMERIKSAAN WIDAL DAN SALMONELLA IgG/ IgM PADA PASIEN SUSPEK DEMAM TIFOID Nurhidayanti; Denny Juraijin; Rafli Wilendra
jurnal kesehatan terapan sains dan teknologi Vol 1 No 1 (2023): Journal Health Applied Science And Technology (JHAST)
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/jhast.v1i1.3

Abstract

Introduction: Typhoid fever is still a very important health problem. This disease is still often found in developing countries located in tropical areas such as Indonesia and subtropical areas. Acute typhoid fever is an acute systemic infectious disease caused by the microorganism Salmonella enteric serotype typhi known as Salmonella typhi (S. typhi). Laboratory examinations to establish the diagnosis of typhoid fever in general can be checked with the Widal test and Salmonella IgG/IgM, tubex test and culture test. Several diagnostic methods are faster, easier to perform and affordable for developing countries with fairly good sensitivity and specificity, such as Widal and salmonella IgG/IgM tests, starting to be used in Indonesia. Purpose: This study was to determine the examination of Widal and salmonella IgG/IgM in patients with suspected typhoid fever.. Research Method: The type of research used was cross sectional, this research was conducted in the Laboratory of Bunda Palembang Hospital. Samples were serum/plasma of patients with suspected typhoid fever. Results: The results showed that there were 11 patients with suspected typhoid fever who were Widal positive and then confirmed using salmonella IgG/IgM and 6 samples were diagnosed positive with typhoid fever. Data analysis using chi-square test obtained p value = 0.014. Conclusion: The conclusion of this study is that there are significant differences in the examination of Widal and salmonella IgG/IgM in patients with suspected typhoid fever
PEMERIKSAAN MIKROSKOPIS KUALITAS SEDIAAN TELUR CACING Trichuris trichiura MENGGUNAKAN METODE NATIF DAN METODE FLOTASI Nurhidayanti; Indah Sari
jurnal kesehatan terapan sains dan teknologi Vol 1 No 2 (2023): Journal Health Applied Science And Technology (JHAST)
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/jhast.v1i2.16

Abstract

Background: Cases of helminthiasis in children are more often infected by Soil Transmitted Helmint (STH) worms, one of which is Trichuris trichiura (whipworm). Stool examination aims to determine the presence or absence of worm eggs or infective larvae. Examination of worm eggs by native and flotation methods is a qualitative examination, each of which has advantages and disadvantages. In the native method, the process is fast, but less sensitive in mild infections. The flotation method has the advantage that the field of view of the microscope is free from faeces so that it is easier to read on a microscope. The drawbacks of the flotation examination are that it takes quite a long time and is more suitable for use in feces that contain small amounts of worm eggs. Purpose: to see the quality of Trichuris trichiura worm egg preparations native and flotation methods. Research Methods: This method is used as an alternative because the native method still has drawbacks, especially in mild infections. This type of research is descriptive analysis. The research was conducted in the Hematology Laboratory of the Muhammadiyah Palembang Institute of Health Sciences and Technology on January 3, 2022 with a total sample of 32 preparations. Results: the study found that the flotation method was more optimal in detecting Trichuris trichiura worm eggs than the native method, with very good quality assessment results on the flotation method 10 preparations of 16 preparations (62.5%), while the native method obtained very good results for 3 preparations of 16 preparations (18.8%). Conclusion: from the research results, there are differences in the results between the flotation method and the native method.