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All Journal International Journal of Cell and Biomedical Science
Sofian Azalia Husain
Stem Cell and Cancer Research (SCCR) Indonesia, Semarang, Indonesia
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Topical Gel of Mesenchymal Stem Cell-Conditioned Medium-induced Serum Injury Accelerates Wound Healing in Skin Excision Tissue Mukti Arja Berlian; Iffan Alif; Prasetyowati Subchan; Frigi Eko Handoyo; Sofian Azalia Husain; Husni Ahmad Sidiq; Dyken Dwi Arlinda; Resanti Adityani
International Journal of Cell and Biomedical Science Vol 1 No 1 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i1.12

Abstract

Introduction: Umbilical cord-derived mesenchymal stem cells (UC-MSCs) accelerating wound closure by increasing VEGF and PDGF level leading to re-epithelialization, cell infiltration, and angiogenesis. It has been found that MSC-conditioned medium (MSC-CM) can enhance migration of fibroblasts in scratch assays. However, the effect of MSC-CM-induced serum injury (MSC-CM-S) formulated in gel to accelerate wound healing remains unclear. This study aims to evaluate the effect of several doses of topical gel of MSC-CM-S in accelerating wound healing. Methods: The MSCs were cultured medium-supplemented serum injury of wounded rat (8:1) to get MSC-CM-S. The topical gel of MSC-CM-S was made by base gel supplemented with MSC-CM-S. Eighteen Wistar rats were randomly assigned into control (C) and treatment groups (T1, T2). Groups were received serum-free medium gel (C), 25 µl MSC-CM-S in topical gel (T1), 50 µl MSC-CM-S in topical gel (T2), twice daily for 9 days. PDGF and VEGF level and fibroblast density were measured by ELISA and HE staining at day 3 and 6, respectively. Results: This study showed that there was significant increase of VEGF and PDGF level along with a significant increase of fibroblast density at day 3 and 6. The T2 showed optimum enhancement level of VEGF, PDGF and fibroblast density. Conclusion: Topical gel of MSC-CM-S was effective to accelerate wound closure by enhancing PDGF and VEGF level in full-thickness skin defect rats.
Co-Authors Dyken Dwi Arlinda Frigi Eko Handoyo Husni Ahmad Sidiq Iffan Alif Mukti Arja Berlian Prasetyowati Subchan Resanti Adityani