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All Journal International Journal of Cell and Biomedical Science
Frigi Eko Handoyo
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Secretome Hypoxia-Mesenchymal Stem Cells Regulate IL-10 Concentrations in STZ-induced Type 1 Diabetes Rats Intan Permatasari Sutrisman; Arini Dewi Antari; Agung Putra; Risky Chandra Irawan; Frigi Eko Handoyo
International Journal of Cell and Biomedical Science Vol 1 No 2 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i2.18

Abstract

Background: Type 1 Diabetic Mellitus (T1DM) is a well-known autoimmune disease that is characterized by a specific adaptative immunity against β-cell antigens. Mesenchymal stem cells (MSC) have emerged as potential immunomodulators in a paracrine manner via their bioactive soluble molecules that involve inflammation-related diseases, including T1DM. Objective: This study aims to investigate the effect of S-HMSC on regulating IL-10 concentrations in STZ-induced T1DM rats. Materials and Methods: This study uses a post-only control group design and randomized system. To induce T1DM rats, an intraperitoneal injection (65 mg/kg BW) of streptozotocin (STZ) was inducted. 20 Wistar rats were subdivided into the following groups: T1DM, DM with 0,5cc S-HMSC (Low-dose), and DM with 1cc S-HMSC (High-dose). The animals received an intraperitoneal injection of S-HMSC once a week for up to 4 weeks. On day 28, the animals were terminated and IL-10 concentrations were measured by ELISA. Results: After S-HMSC administration, the concentration of IL-10 in the treated group was increased in either low-dose or high-dose groups compared with the T1DM group. Conclusion: Administration of secretome-hypoxia MSC may regulate IL-10 concentrations in STZ-induced Type 1 Diabetes Rats.
The Effect of Hypoxia on the Soluble Molecules of Human Umbilical Cord-Derived Mesenchymal Stem Cells (UC-MSCs) Agus Widyatmoko; Iffan Alif; Risky Candra Satria Irawan; Frigi Eko Handoyo; Husni Ahmad Sidiq
International Journal of Cell and Biomedical Science Vol 1 No 3 (2023)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i3.24

Abstract

Background: Umbilical cord-derived stem cells (UC-MSCs) are essential cell sources for cell-based therapies in regenerative medicine. Hypoxia is a key element of the stem cell niche and crucial for the preservation of UC-MSCs properties. The normal growth conditions for UC-MSCs are under atmospheric oxygen concentrations of 20–21%. However, the administration of UC-MSCs in inflammatory conditions provides oxygen-deficient environments. Thus, treating UC-MSCs with low oxygen exposure provides them with more survival and recovery potential. Objective: In this study, we assessed the impact of hypoxia incubation for 12 h on the UC-MSCs proteome. Methods: UC-MSCs were isolated from UC patients regarding informed consent. At passage 5, in 80% confluent, UC-MSCs were incubated in 5% O2 for 12 h. The morphology of UC-MSCs was assessed using a microscope. The level of FGF-2, FGF-8, TNF-α, and HSP-70 were analyzed using ELISA. Results: Hypoxic condition could change their morphology and enhance the cellular density compared to normoxic conditions in vitro. The level of FGF-2, FGF-8, TNF-α, and HSP-70 were significantly increased after the hypoxic condition of UC-MSCs compared to normoxia. Conclusion: Our findings suggest that the hypoxic condition was able to induce survival capacity and soluble molecules secreted by UC-MSCs.
Co-Authors Agung Putra Agus Widyatmoko Arini Dewi Antari Husni Ahmad Sidiq Iffan Alif Intan Permatasari Sutrisman Risky Candra Satria Irawan Risky Chandra Irawan