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All Journal Journal of Multidisciplinary Science: MIKAILALSYS Asian Journal of Science, Technology, Engineering, and Art
Mohamed Wuna
Federal University of Technology, Minna, Nigeria
Agriculture, Biological Sciences & Forestry Arts Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Engineering Environmental Science Physics Social Sciences Other

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Antibacterial Activities and Synergistic Effect of the Bioactive Compounds of Selected Medicinal Plants Against Diarrhoea-Causing Pathogens Murtala Saadu; Nasiru Usman Adabara; Faruk Abubakar Kuta; Hadiza Lami Muhammed; Mohamed Wuna
Asian Journal of Science, Technology, Engineering, and Art Vol 1 No 1 (2023): OCTOBER
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajstea.v1i1.1818

Abstract

In this study, the antimicrobial properties and toxicological effects of Anogeissus leiocarpus and Khaya senegalensis, two traditional medicinal plants from West Africa, were investigated against diarrhea-causing pathogens. Cold maceration was used to prepare extracts from the plants. Anogeissus leiocarpus yielded 22.87g of extract, while Khaya senegalensis yielded 13.94g. Both plant crude extracts exhibited varying degrees of antibacterial activity against Vibrio cholerae, Klebsiella pneumoniae, and Salmonella enterica serovar at different concentrations. A. leiocarpus and K. senegalensis showed the highest antibacterial activity, with significantly higher zones of inhibition at all concentrations against all test organisms. The Minimum Inhibitory Concentration (MIC) for A. leiocarpus ranged from 0.10 to 0.96 mg/mL, while the Minimum Bactericidal Concentration (MBC) ranged from 0.10 to 1.09 mg/mL. For K. senegalensis, MIC ranged from 0.96 to 1.80 mg/mL, and MBC ranged from 1.02 to 1.92 mg/mL. Fractionation of the most active crude extracts resulted in the highest yields in the n-Hexane fractions for both A. leiocarpus and K. senegalensis. Significant differences were observed in the antibacterial activity of these fractions. K. senegalensis fractions and A. leiocarpus n-Hexane fraction showed the highest activity against V. cholerae, while the ethyl acetate fraction of K. senegalensis exhibited significant activity against K. pneumoniae. The aqueous fraction of A. leiocarpus displayed the highest activity against Salmonella, whereas none of the K. senegalensis fractions were active against Salmonella. Antibacterial activity of K. senegalensis ethyl acetate and A. leiocarpus n-Hexane and aqueous column chromatography fractions against the test organisms was concentration-dependent, with the highest antimicrobial activity observed at 200 mg/mL concentration. Importantly, there were no significant differences in the body weights of experimental animals across all groups. In conclusion, A. leiocarpus and K. senegalensis extracts and their fractions demonstrated promising antimicrobial properties against diarrhea-causing pathogens. These findings support their traditional medicinal use in West Africa and suggest their potential as natural remedies for combating bacterial infections.
In Vitro Antibacterial Activities of Selected Medicinal Plants Against Diarrhoea Causing Pathogens Murtala Saadu; Faruk Abubakar Kuta; Hadiza Lami Muhammed; Mohamed Wuna
Journal of Multidisciplinary Science: MIKAILALSYS Vol 1 No 2 (2023): AUGUST
Publisher : Lembaga Yasin AlSys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/mikailalsys.v1i2.1650

Abstract

Diarrhea has been a major cause of death, especially among children in developing countries. Traditional preparations of medicinal plants with antimicrobial activities have been extensively used in West African regions. This study was conducted to determine the antibacterial activities of selected medicinal plants against pathogens that cause diarrhea. Fresh leaves of three medicinal plants, namely Anogeissus leiocarpus, Vernonia amygdalina, and Piliostigma thonningii, were collected in Minna, Niger State. Pathogens causing diarrhea (Vibrio cholerae, Klebsiella pneumoniae, and Salmonella enterica serovar) were isolated and identified from stool samples of individuals suffering from diarrhea. The plant material was extracted using the maceration technique with methanol. The antibacterial effectiveness of the selected plant extracts was evaluated using the agar well diffusion method on Muller Hinton agar. The minimum inhibitory concentration was determined using the Broth tube dilution method, while the minimum bactericidal concentration was determined by plating out on nutrient agar plates with no visible growth. The results indicated that all plant extracts effectively suppressed the microbial growth of diarrhea-causing bacteria with varying potency. Among them, the extract of Anogeissus leiocarpus consistently exhibited the largest inhibitory zone diameters across all three bacterial strains at a concentration of 300 mg/mL (Vibrio cholerae: 32.33 mm, Klebsiella pneumoniae: 34.00 mm, and Salmonella enterica serovar Kentucky: 36.33 mm). This extract can be considered the most active. For Anogeissus leiocarpus, the MIC values ranged from 0.19 to 0.96 mg/mL, and the MBC values ranged from 0.20 to 1.09 mg/mL. For Vernonia amygdalina, the MIC values ranged from 0.96 to 1.80 mg/mL, and the MBC values ranged from 1.20 to 1.920 mg/mL. The results of this study validate the traditional use of these plants in medicine. However, further studies, including the isolation and identification of active compounds would be necessary to fully understand the mechanisms behind the observed antimicrobial effects.
Co-Authors Faruk Abubakar Kuta Hadiza Lami Muhammed Murtala Saadu Murtala Saadu Nasiru Usman Adabara