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All Journal Asian Journal of Health and Applied Sciences
Purwaeni, Purwaeni
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Analysis of Caffeine Levels in Packaged Coffee in Indonesia Baehaki, Farhan; Nurhamidah, Yeni; Purwaeni, Purwaeni
Asian Journal of Health and Applied Sciences Vol. 2 No. 1 (2023): Asian Journal of Health and Applied Sciences (AJHAS)
Publisher : Lighthouse Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53402/ajhas.v2i1.238

Abstract

Coffee is a popular and much-loved drink in Indonesian society to relieve drowsiness or increase stamina when working. This is because in coffee, there is an active compound, namely caffeine. Although it has benefits, in fact, caffeine will have a negative impact on the body if consumed in excess. The Indonesian National Standard 01-3542-2004 has determined that the limit value of caffeine content in food and beverages is 0.45–2% w/w. This study aimed to determine the caffeine content of packaged coffee sold in the community using a UV-Vis Spectrophotometer. The results of the analysis showed that 16 of the 20 samples analyzed had caffeine levels in accordance with the provisions of the Indonesian National Standard, while the other 4 samples had caffeine levels above the specified value. Even sample Q has a caffeine content of 7.12% w/w, far above the threshold value. Based on the results of this study, it can be concluded that there are still packaged coffees containing caffeine above the specified threshold value. So, consumers should pay attention to the amount of caffeine consumed so as not to cause a negative impact on their health.
The Effect of Garlic Decoction (Allium sativum L.) on The Histological Features of The Liver of Alloxan-induced Mice (Mus musculus) Kodariah, Liah; Karimah, Haniefatul; Purwaeni, Purwaeni; Fadilah, Tyas Ismi
Asian Journal of Health and Applied Sciences Vol. 2 No. 2 (2023): Asian Journal of Health and Applied Sciences (AJHAS)
Publisher : Lighthouse Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53402/ajhas.v2i2.280

Abstract

Giving alloxan can cause disruption of insulin production resulting in hyperglycemia. Hyperglycemia causes Reactive Oxygen Species (ROS) resulting in oxidative stress and liver cell damage. The use of natural ingredients, one of which is garlic (Allium sativum L) in the form of boiled water, because it contains flavonoid and organosulfur compounds. Therefore, the purpose of this study was to determine the histology of the liver of mice (Mus musculus) induced by alloxan after being given a decoction of garlic (Allium sativum L). This study used experimental research with a completely randomized design method on mice consisting of 6 groups with 4 replications each. The groups in this study consisted of a negative control group (without treatment), a positive control group (alloxan injection) a comparison control group (alloxan injection + glibenclamide) treatment groups at doses I, II and III (alloxan-induced + garlic boiled water (Allium sativum L) respectively 20 gr, 40 gr and 60 gr). Histology of the liver of mice (Mus musculus) was made by paraffin method with Hematoxylin Eosin (HE) staining. The result showed that there was a significant difference due to the value (p<0,05) but for a KP and P3 there was no significant difference with the value (p>0,05) on hydropic degeneration. Based on the results of the study, it can be concluded that the decoction of garlic (Allium sativum L) has an effect on the alloxan-induced liver histology of mice (Mus musculus).
Co-Authors Fadilah, Tyas Ismi Farhan Baehaki Karimah, Haniefatul Liah Kodariah Nurhamidah, Yeni