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All Journal Indonesian Journal of Multidisciplinary Science International Journal of Islamic Education, Research and Multiculturalism (IJIERM)
Wiwik Handayani
Center for Agro-industrial Research, Badan Riset dan Inovasi Nasional, West Java
Religion Aerospace Engineering Arts Humanities Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Engineering Environmental Science Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Mathematics Public Health Social Sciences Other

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Coffee cherry processing waste to produce value-added products through clean production biorefineries Silvia Oktavia Nur Yudiastuti; Wiwik Handayani; Elok Kurnia Novita Sari; Rizza Wijaya; Aulia Brilliantina; Ahmad Haris Hasanuddin Slamet
Indonesian Journal of Multidisciplinary Science Vol. 3 No. 3 (2023): Indonesian Journal of Multidisciplinary Science
Publisher : International Journal Labs

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55324/ijoms.v3i3.779

Abstract

Indonesia is the 4th largest coffee-producing country in the world. Coffee cherry can produce 60% CCPW, more than the core product of coffee beans as the main product produced. CCPW contains lignocellulosic material that can be converted into value-added products. This research optimizes the production of xylose and xylanase enzymes as an integrated process with a biorefinery model to increase the added value of CCPW using the Research Surface Methodology (RSM). Enzyme production is carried out using a bioprocess using Trichoderma viride. Xylose production is carried out using the dregs of xylanase production as raw material and xylanase enzymes which are produced in the integrated process. The xylanase and xylose produced are in the liquid phase. The treatment design for xylanase production is substrate concentration and incubation time, while for xylose production it is substrate concentration and xylanase concentration. Based on the optimization conducted, the optimum condition of xylanase production was 75% substrate concentration with an incubation time of 72 hours, which will result in xylanase an enzyme with an activity of 29.275 U/ml with desirability of 99.55%. On the other hand, the optimum condition of xylose production was 12.54% substrate concentration with a xylanase concentration of 4.01%, which will result in 0.53 g/L xylose content with desirability of 94.06%. These findings show that xylose production efficiency can be achieved through integrated two-stage biorefinery production for efficient and sustainable utilization of CCPW.
The utilization of Trichoderma viride in Optimising Xylanase Production from Coffee Cherry Processing Waste Silvia Oktavia Nur Yudiastuti; Wiwik Handayani; Elok Kurnia Novita Sari; Rizza Wijaya; Aulia Brilliantina; Ahmad Haris Hasanuddin Slamet
International Journal of Islamic Education, Research and Multiculturalism (IJIERM) Vol 6 No 1 (2024): IJIERM Edition January
Publisher : The Islamic Education and Multiculturalism Foundation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47006/ijierm.v6i1.298

Abstract

The use of enzymes in Indonesia is increasing rapidly and is used in various industries, especially FCMG. One of the enzymes that is widely used is xylanase, which is an extracellular enzyme that can hydrolyze xylan into xylose and xylo-oligosaccharides. This research aims to determine the optimum conditions for the xylanase production process from coffee cherry processing waste (CCPW) using Trichoderma viride through solid fermentation. The research method used was experimental using central composite design (CCD) in Design Expert 13.0 software. The treatment designs used were fermentation times of 24 hours, 48 ​​hours, and 72 hours; and substrate concentrations of 25%, 50%, and 75%. The experimental response analysis design was enzyme activity, protein content, and specific activity. The results of the research show that the optimum conditions for xylanase production are at a substrate concentration of 75% and a fermentation time of 24 hours with a desirability value of 0.742, which produces an enzyme activity value of 36.388 U/mL and a dissolved protein content of 0.494 mg/mL with a specific activity of 73.660. U/mg. The verification results show conformity with the formula predicted by the Design Expert 13.0 software, namely enzyme activity of 36,292 U/mL and protein content of 0.488 mg/mL with a specific activity of 74,369 U/mg
Co-Authors Ahmad Haris Hasanuddin Slamet Ahmad Haris Hasanuddin Slamet Brilliantina, Aulia Elok Kurnia Novita Sari Elok Kurnia Novita Sari Rizza Wijaya Rizza Wijaya Silvia Oktavia Nur Yudiastuti