Article Per Year (5 Year)
p-Index From 2019 - 2024
0.23
P-Index
This Author published in this journals
All Journal Jurnal Bahana Kesehatan Masyarakat (Bahana of Journal Public Health)
Indah Puspita
Medical Technology Laboratory Polytechnic of Health Ministry of Health Jakarta III
Dentistry Environmental Science Medicine & Pharmacology Nursing Public Health

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
PENGGUNAAN HOTPLATE SUHU 60°C SELAMA 10 MENIT SEBAGAI ALTERNATIF PEMANASAN PROSES FIKSASI SEDIAAN HISTOLOGI Burhannudin Burhannudin; Warida Warida; Indah Puspita
Jurnal Bahana Kesehatan Masyarakat (Bahana of Journal Public Health) Vol 7 No 2 (2023): Jurnal Bahana Kesehatan Masyarakat (Bahana of Journal Public Health)
Publisher : Poltekkes Kemenkes Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35910/jbkm.v7i2.683

Abstract

Background: The fixation process in histological preparation has been shortened by using microwave heating at 60°C for 2-10 minutes while maintaining the quality. However, the use of hotplates in this process is still limited. The study aims to determine the optimal duration of hotplate heating at 60°C for fixation. Method: An experimental study was conducted using a post-test-only control group design. The study includes 20 histological specimens from two control groups (conventional and microwave) and three treatment groups with hotplate heating durations of 3 minutes (W1), 5 minutes (W2) and 10 minutes (W3). Histological slides were evaluated based on staining results and scored using a scoring system (0-3). The scoring was tested using the Kruskal-Wallis and Mann-Whitney tests, with a significance level of p<0.05. Result: microscopic evaluation of the specimens revealed dark staining with Haematoxylin and Eosin in the W2 and W3 groups. However, the W1 group showed similar results (p>0.05) compared to the control group, while the W2 and W3 groups indicated significantly different results (p<0.05) compared to the control group. Conclusion: using a hotplate at 60°C for 10 minutes effectively accelerated the fixation process without compromising the quality of Haematoxylin and Eosin staining in histological specimens.
Co-Authors Burhannudin Burhannudin Warida Warida