The aim of this study was to determine the effect of pituitary extract on estrous characteristics and the percentage of pregnancy in sheep. This study used a sample of 18 individuals. This study used 3 treatments 6 replications. The treatment used was the control treatment by giving 1 ml of PGF2α. Treatment 1 is by administering 1 ml of PGF2α, 1;0.5;0.25 ml of pituitary extract and 1 ml of PGF2α. Treatment 2 is by administering 1 ml of PGF2α, 2;1;0.5 ml of pituitary extract and 1 ml of PGF2α. The results showed that there was a very significant difference in the use of pituitary extract, this could affect the onset of estrus 0.00 (P<0.01), and there was also a significant difference (P<0.05) in the intensity of estrus.