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Sitaresmi, Sitaresmi
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PENGEMBANGAN DATABASE MIKROORGANISME INDIGENOS INDONESIA Sjamsuridzal, Wellyzar; Oetari, Ariyanti; Hertono, Gatot F.; Sitaresmi, Sitaresmi
Makara Journal of Science Vol. 10, No. 1
Publisher : UI Scholars Hub

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Abstract

Development of Database for Indigenous Indonesian Microorganisms. The objective of the research is to create and develop a database of indigenous Indonesian microorganisms based at the University of Indonesia. Development of the database of indigenous Indonesian microorganisms was carried out in several stages, i.e. data identification, database design, programming, data entry, testing and debugging, and repairing and maintenance. Development of the database utilized the licensed software of General Public License (GPL), which include Linux RedHat 9.0 (operating system), Apache ver. 2.20 (web server), MySQL ver. 4.2 (database server), and PHP ver. 4.3 (web interface programming language). The result of this research is a database named UI Bioinfo which has the following facilities: online catalog search for UICC (University of Indonesia Culture Collection) strains collection and sequence homology search utility through BLAST (Basic Local Alignment Search Tool). Integrated information on strains collection was first carried out on the yeast collection. At present, UI Bioinfo contains information for 297 strains that includes isolation data, morphological descriptions, physiology-biochemical characteristics, and images. Moreover it also contains sequence data from the large subunit (LSU) ribosomal RNA gene and the internal transcribed spacer (ITS) regions. UI Bioinfo can be accessed from the following site: http://152.118.162.250/bio/. Future development will be addition of data from the other collections in UICC
PENGEMBANGAN DATABASE MIKROORGANISME INDIGENOS INDONESIA Sjamsuridzal, Wellyzar; Oetari, Ariyanti; Hertono, Gatot F.; Sitaresmi, Sitaresmi
Makara Journal of Science Vol. 10, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Development of Database for Indigenous Indonesian Microorganisms. The objective of the research is to create and develop a database of indigenous Indonesian microorganisms based at the University of Indonesia. Development of the database of indigenous Indonesian microorganisms was carried out in several stages, i.e. data identification, database design, programming, data entry, testing and debugging, and repairing and maintenance. Development of the database utilized the licensed software of General Public License (GPL), which include Linux RedHat 9.0 (operating system), Apache ver. 2.20 (web server), MySQL ver. 4.2 (database server), and PHP ver. 4.3 (web interface programming language). The result of this research is a database named UI Bioinfo which has the following facilities: online catalog search for UICC (University of Indonesia Culture Collection) strains collection and sequence homology search utility through BLAST (Basic Local Alignment Search Tool). Integrated information on strains collection was first carried out on the yeast collection. At present, UI Bioinfo contains information for 297 strains that includes isolation data, morphological descriptions, physiology-biochemical characteristics, and images. Moreover it also contains sequence data from the large subunit (LSU) ribosomal RNA gene and the internal transcribed spacer (ITS) regions. UI Bioinfo can be accessed from the following site: http://152.118.162.250/bio/. Future development will be addition of data from the other collections in UICC
PENGEMBANGAN DATABASE MIKROORGANISME INDIGENOS INDONESIA Sjamsuridzal, Wellyzar; Oetari, Ariyanti; Hertono, Gatot F.; Sitaresmi, Sitaresmi
Makara Journal of Science Vol. 10, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Development of Database for Indigenous Indonesian Microorganisms. The objective of the research is to create and develop a database of indigenous Indonesian microorganisms based at the University of Indonesia. Development of the database of indigenous Indonesian microorganisms was carried out in several stages, i.e. data identification, database design, programming, data entry, testing and debugging, and repairing and maintenance. Development of the database utilized the licensed software of General Public License (GPL), which include Linux RedHat 9.0 (operating system), Apache ver. 2.20 (web server), MySQL ver. 4.2 (database server), and PHP ver. 4.3 (web interface programming language). The result of this research is a database named UI Bioinfo which has the following facilities: online catalog search for UICC (University of Indonesia Culture Collection) strains collection and sequence homology search utility through BLAST (Basic Local Alignment Search Tool). Integrated information on strains collection was first carried out on the yeast collection. At present, UI Bioinfo contains information for 297 strains that includes isolation data, morphological descriptions, physiology-biochemical characteristics, and images. Moreover it also contains sequence data from the large subunit (LSU) ribosomal RNA gene and the internal transcribed spacer (ITS) regions. UI Bioinfo can be accessed from the following site: http://152.118.162.250/bio/. Future development will be addition of data from the other collections in UICC
AKTIVITAS LIPOLITIK Rhizopus microsporus var. rhizopodiformis ISOLAT UICC No. 6 Iman, Iman; Sitaresmi, Sitaresmi; Prayudi, Doni; Kudin, Kudin
Makara Journal of Science Vol. 6, No. 3
Publisher : UI Scholars Hub

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Abstract

The lipolytic activity of Rhizopus microsporus var rhizopodiformis isolat UICC No. 6. A study was carried out to examine the effect of peptone concentration (2,5% or 5%), inoculum concentration (0,1%, 0,5% or 1%) and incubation period (0 – 96 hours, interval 12 hours) on the lipolytic activity of Rhizopus microsporus var rhizopodiformis isolat UICC No. 6. Fermentation was done using the basal medium from Samad and the lipolytic activity on olive oil substrate was measured employing titration method with 0,05M NaOH. Lipolytic activity is expressed as unit/ml and one unit is defined as 1 mmol fatty acid liberated per minute. Results show that optimum lipolytic activity was obtained from 1 % inoculum, 5% peptone after 72 hours incubation period
AKTIVITAS LIPOLITIK Rhizopus microsporus var. rhizopodiformis ISOLAT UICC No. 6 Iman, Iman; Sitaresmi, Sitaresmi; Prayudi, Doni; Kudin, Kudin
Makara Journal of Science Vol. 6, No. 3
Publisher : UI Scholars Hub

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Abstract

The lipolytic activity of Rhizopus microsporus var rhizopodiformis isolat UICC No. 6. A study was carried out to examine the effect of peptone concentration (2,5% or 5%), inoculum concentration (0,1%, 0,5% or 1%) and incubation period (0 – 96 hours, interval 12 hours) on the lipolytic activity of Rhizopus microsporus var rhizopodiformis isolat UICC No. 6. Fermentation was done using the basal medium from Samad and the lipolytic activity on olive oil substrate was measured employing titration method with 0,05M NaOH. Lipolytic activity is expressed as unit/ml and one unit is defined as 1 mmol fatty acid liberated per minute. Results show that optimum lipolytic activity was obtained from 1 % inoculum, 5% peptone after 72 hours incubation period
Co-Authors ARIYANTI OETARI Hertono, Gatot F. Iman Iman, Iman Kudin, Kudin Prayudi, Doni WELLYZAR SJAMSURIDZAL