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Lukas Pardosi
Biology Study Program, Faculty of Agriculture, Science and Health, University of Timor Jalan Km. 09, Sasi Village, Kefamenanu City District, North Central Timor Regency, East Nusa Tenggara Province, Indonesia, 85613
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education

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Isolasi Dan Uji Aktivitas Bakteri Proteolitik Yang Berasosiasi Dengan Spons Stylotella Sp. Dan Potensinya Sebagai Antibakteri Mariana Septiani Seuk; Lukas Pardosi; Gergonius Fallo; Adelya Irawan Manalu
BIOEDUSCIENCE Vol 8 No 1 (2024): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/12980

Abstract

Background: The province of East Nusa Tenggara (NTT), Indonesia, boasts a significant expanse of marine territory, making its waters a potential treasure trove of aquatic resources. One such marine resource is the Stylotella SP sponge, which is found in this region. Sponges like Stylotella SP often have intricate relationships with microorganisms, including bacteria. Method: This research was to characterize and identify proteolytic bacteria and test bioactive compounds from isolates of proteolytic bacteria from the sponge Stylotella sp. Proteolytic bacterial strains were rejuvenated first in 2% TSA + Nacl medium and then characterized microscopically and biochemically using catalase and oxidase tests. The diffusion method used the antibacterial activity test against E.coli and S. aureus. Result: The results showed that proteolytic bacterial isolates had irregular and rounded colony shapes, wavy and intact colony edges, hilly and convex colony elevation, and white color, while biochemically, it was declared positive for the catalase and oxidase tests. Antibacterial activity tests on proteolytic bacterial strains showed the ability to inhibit the growth of Staphylococcus aureus with an inhibitory capacity of 9.5 mm and Escherichia coli with an inhibitory capacity of 7 mm.
Co-Authors Adelya Irawan Manalu Gergonius Fallo Mariana Septiani Seuk