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All Journal JTCST (Journal of Tropical Crop Science and Technology)
Agus Zainudin
Universitas Muhammdiyah Malang
Agriculture, Biological Sciences & Forestry

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Effect Of Indole Butyric Acid (Iba) And Thidiazuron (Tdz) Concentrations On Explant Growth Planlet Porang (Amorphophallus Muelleri Blume.) In Vitro Culture Farhan Chairul Haq; maftuchah; Agus Zainudin
Journal of Tropical Crop Science and Technology Vol. 4 No. 2 (2022): VOLUME 4, NO. 2, OCTOBER 2022
Publisher : Universitas Muhammadiyah Malang

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Abstract

Porang (Amorphophallus muelleri Blume.) is a type of tuber plant that is widely available in Indonesia and has tremendous potential and prospects to be developed in Indonesia. Porang is widely exported from Indonesia to several countries. The demand for porang exports continues to increase while the production of porang is still low. In vitro culture is an alternative solution in porang plant propagation to meet the needs of many porang seeds and in a relatively short period of time. Provision of growth regulators in the media can affect the growth of porang. This study aims to obtain information whether there is an interaction between IBA and TDZ in the media, to obtain information on the effect of adding IBA and TDZ with different concentrations on the growth of porang plantlets. This research was conducted using MS medium which was then added with IBA with a concentration of 0.5 ppm, 1 ppm and added ZPT TDZ with a concentration of 0.5 ppm, 1 ppm and 1.5 ppm. The results of this study showed that there was no significant difference between the emergence of shoots and the time of emergence of leaves from all treatments. The I1T3 treatment (IBA 0.5 ppm + TDZ 1.5 ppm) was the best combination treatment in this study by showing the fastest root emergence time (6 MSI), the highest average number of shoots (7.25 shoots), the average number of shoots the highest number of roots (3.67 roots), the highest average number of leaves (6 strands) and the highest average shoot height (1.33 cm).
Effect Of Seed Soaking Time In Kno3 On Germination, Vegetative Morphology, And Results Of Bambara Chicken (Vigna Subterranea (L.) Verdcourt) Aulia Ika Sari; Aulia Zakiya; Agus Zainudin
Journal of Tropical Crop Science and Technology Vol. 5 No. 1 (2023): VOLUME 5, NO. 1, MARCH 2023
Publisher : Universitas Muhammadiyah Malang

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Abstract

The The bambara groundnut has the potential to continue to be developed, but the obstacle faced is the very limited availability of bambara groundnut seeds. This is because there are no varieties from the results of breeding that are released and the low quality of the seeds. Low seed quality is caused by slow germination and results in the seed growth process being non-unison, this is influenced by seed dormancy. The purpose of this study was to determine the effect of soaking time in KNO3 on germination, vegetative morphology, and crop yield in the field by measuring seed viability and vigor, vegetative morphology and yield. The research was carried out for 4 months, from June-October 2022 which took place at the Agrotechnology Laboratory of the Muhammadiyah University of Malang and Dadaprejo Land, Batu City. The study was conducted using a simple Completely Randomized Block Design (RKLT) consisting of 10 treatments, and was repeated 3 times. The observed variables included moisture content, DB, PTM, IV, KCT, T50, plant height, number of leaves, leaf shape, leaf color, stem color, pod color, pod shape, number of pods, pod dry weight, number of seeds, dry weight seeds, yield potential, and weight of 1000 seeds. The results showed that the long soaking of seeds in KNO3 did not affect their vegetative morphology. Soaking time of seeds in KNO3 had no significant effect on DB and PTM values, but had a very significant effect on IV values, the number of leaves in the vegetative and generative phases. And it has a significant effect on the height of the KCT, T50, and plant height in the vegetative phase. As for the yield variable, the results showed no significant effect. Soaking time in KNO3 only affected germination, plant height, and number of bambara groundnut leaves.
Optimization Of Callus Induction Using A Combination Of 2,4 Dichlorophenoxy Acetic Acid And Sitokinin On Pineapple (Caisim) Cales In Vitro Agnes Putri Ayundaris; Fatimah Nursandi; Agus Zainudin; machmudi; Erny Ishartati
Journal of Tropical Crop Science and Technology Vol. 6 No. 1 (2024): VOLUME 6, NO. 1, MARCH 2024
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jtcst.v6i1.32887

Abstract

In Indonesia, pineapple is one of the horticultural commodities that has the potential to be developed. It is seen that the amount of demand for fresh pineapple from abroad is quite high. Intensification of pineapple land is still lacking where the number of pineapple seedlings planted has only reached 2,500 from ideally 10,000 stems per hectare. Alternative to overcome this problem is by propagating plants in vitro through callus induction which later is thought to potentially have quality and quantity seeds as expected. This study uses a combination of ZPT 2,4-D and cytokines which are expected to grow callus with good quality and relatively faster time.This study uses Factorial Randomized Block Design (RBD). By using the first factor namely 2,4-D concentration and the second factor cytokines (BAP and TDZ). The data can be analyzed for variance and a real honest test (BNJ) level of 5%. Based on the results of the study, it was found that the combination of giving concentrations to 2,4-D and cytokines was not significant for each parameter. The interaction between 2,4-D growth regulators and cytokinins has no significant effect on the induction of pineapple varieties of Smoooth cayenne. The treatment of 2,4-D 0 mg/L - 5 mg/L growth regulators has no significant effect on callus induction in vitro on the Smoooth cayenne varieties. The treatment of cytokinin (Thidiazuron 0.001 mg/L - 0.1 mg/L and BAP 0 mg/L - 4 mg/L) had no significant effect on in vitro callus induction on the Smoooth cayenne varieties.
The Effect Of Shoot Cuttings And Vermiwash Concentration On Orchid Growth (Onchidium sphacelatum Lindl.) Aeroponically Ibrahim; Untung Santoso; Agus Zainudin; Padhina Pangestika
Journal of Tropical Crop Science and Technology Vol. 6 No. 1 (2024): VOLUME 6, NO. 1, MARCH 2024
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jtcst.v6i1.32984

Abstract

The Oncidium sphacelatum orchid, commonly propagated vegetatively through pseudobulb separation, presents opportunities for further research into propagation methods and organic fertilizer application. This study, conducted in Batu City, aimed to explore the interaction between vermiwash concentration and shoot number on aeroponically cultivated Orchid sphacelatum Lind cuttings, as well as individual treatment effects. Employing a factorial RKLT design with shoot number (one or two) and vermiwash concentration (100, 200, or 300 ml/L) as factors, the study evaluated plant height, leaf count, stem length, shoot length, root count, and root length as variables. Data analysis involved ANOVA and BNJ tests. Results revealed that cuttings with two shoots and a 200 ml/L vermiwash concentration promoted optimal leaf growth. The number of shoots influenced shoot length, with two shoots demonstrating superior growth compared to single shoot cuttings. Interestingly, vermiwash concentration did not significantly impact shoot growth parameters, indicating that its effects may be limited in this context. This study sheds light on the potential benefits of using vermiwash in orchid cultivation, particularly in enhancing leaf growth when combined with multiple shoot cuttings. However, further investigation is needed to understand the nuanced effects of vermiwash on other growth aspects and its interaction with different propagation methods.Overall, the findings contribute to the optimization of orchid propagation techniques, providing valuable insights for orchid growers seeking to enhance plant growth and productivity sustainably. Further research could delve deeper into the mechanisms underlying the observed effects and explore additional variables to refine cultivation practices for Oncidium sphacelatum orchids.
Co-Authors Agnes Putri Ayundaris Aulia Ika Sari Aulia Zakiya Erny Ishartati Farhan Chairul Haq Fatimah Nursandi Ibrahim machmudi maftuchah Padhina Pangestika Untung Santoso