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Thomas Mata Hine
Faculty of Animal Husbandry, Marine and Fisheries, Nusa Cendana University

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Pengaruh Penambahan Fruktosa dalam Pengencer Natrium Klorida Fisiologis Kuning Telur Ayam Ras terhadap Kualitas Spermatozoa Babi Landrace Novaldy Imanuel Toto; Wilmientje M. Nalley; Aloysius Marawali; Thomas Mata Hine
Animal Agricultura Vol 2 No 1 (2024): Animal Agricultura
Publisher : Yayasan Sumber Daya Manusia Cerdas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59891/animacultura.v2i1.62

Abstract

This study aims to determine the effect of adding fructose to egg yolk diluent on the quality of Landrace pig spermatozoa. This research used a completely randomized design (CRD) method with 6 treatments and 5 replications. The following are the various treatments, namely: T0=NaCl fis 2.4 mL + TY 0.6 grams + 0 grams of fructose, T1= NaCl fis 2.4 mL + TY 0.6 grams + 0.015 grams of fructose, T2= NaCl fis 2 .4 mL + TY 0.6 gram + 0.030 gram fructose, T3= NaCl fis 2.4 mL + TY 0.6 gram + 0.045 gram fructose, T4= NaCl fis 2.4 mL + TY 0.6 gram + 0.060 gram fructose, and T5 = NaCl fis 2.4 mL + TY 0.6 grams + 0.075 grams of fructose. Good quality cement is diluted using treatment diluent, evaluated post-dilution, packaged in ependop then stored in a cool box with a temperature of 18-20°C. Evaluation of spermatozoa motility, viability, abnormalities and survival is carried out every 8 hours. The research results obtained showed that the 24th hour of storage with treatment T0 (47.00 ± 10.95), T1 (51.00 ± 7.42), T2 (55.00 ± 6.12), T3 (54.00 ±4.18), T4 (50.00±3.54), and T5 (44.00±4.18) were able to stay above 40% which is feasible for IB. Statistical tests showed that the treatments did not significantly differ on the quality of landrace pig spermatozoa. It was concluded that the semen quality of landrace pigs could be optimally improved by using a fructose level in the diluent of 0.30 grams.
Pengaruh Penambahan Ekstrak Biji Kelor Kering (Moringa oleifera Lam) dalam Pengencer Beltsville Thawing Solution terhadap Kualitas Semen Cair Babi Landrace Fransiska Onya Pandahuki; W. Marlene Nalley; Kirenius Uly; Thomas Mata Hine
Animal Agricultura Vol 2 No 1 (2024): Animal Agricultura
Publisher : Yayasan Sumber Daya Manusia Cerdas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59891/animacultura.v2i1.67

Abstract

The aim of this research was to determine the effect of adding dry moringa seed extract (DMSE) added to the Beltsville thawing solution (BTS) diluent on the quality of landrace boar semen during storage. This research used experimental methods and a completely randomized design  consisting of 5 treatments and 5 replications to form 25 experimental units. The treatment in question was : T0 = BTS EY + 0% DMSE, T1 = BTS EY + 1% DMSE, T2 = BTS EY + 2% DMSE, T3 = BTS EY + 3% DMSE, T4 = BTS EY + 4% DMSE. This diluted semen will be stored at a temperature of 18-20ºC and evaluation of motility, viability, abnormalities and survival of spermatozoa was carried out every 8 hours of storage. Data was analysis of the average, standard deviation, variance and continued with the Duncan test. The results showed that T2 treatment with a 2% DMSE level at 32 hours of storage gave the best results (P<0.05) compared to other treatments, with motility values (51, 80±4.71), viability (65.54±5.00), abnormality (5.81±0.81 and survival value of (38.89±1.21). It was concluded that the addition of EBKK at a level of 2% to the BTS diluent provided a good response in maintaining motility, viability and abnormalities.