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All Journal Majalah Kedokteran Gigi Indonesia Dental Journal (Majalah Kedokteran Gigi)
Putri, Tansza Setiana
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Dentistry

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Characterization of β-tricalcium phosphate derived from green mussel shells (Molarity variation) Santoso, Hillary Aurenne; Eddy, Eddy; Kesnatri, Manuel Romario; Putri, Tansza Setiana; Dipankara, Jackson; Nugroho, Astri Rinanti; Thi, Bang Le
Majalah Kedokteran Gigi Indonesia Vol 9, No 3 (2023): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.89690

Abstract

β-Tricalcium phosphate (β-TCP) is a widely used bioceramic material. In dentistry, it is commonly used as bone graft material. β-TCP is osteoconductive, bioresorbable, bioactive, and has biocompatibility properties. This study aims to evaluate the optimum molarity of CaO and H3PO4 to synthesize β-TCP from a natural source (Perna viridis linn). This is laboratory experimental research conducted by reacting calcium compounds from green mussel shells and phosphoric acid using the dissolution precipitation method with variations in molarity ratio. X-ray diffraction (XRD), scanning electron microscope (SEM), and fourier transform infrared (FTIR) were used to identify the characteristics of β-TCP synthesized from green mussel shells. The XRD chart pattern showed the formation of peaks identical to the β-TCP (Sigma-Aldrich). However, formation of whitlockite phase was also seen in the results. FTIR results showed that phosphate, hydroxyl, and carbonyl groups were shown on the graph and could be identified as β-TCP. SEM characterization showed that the sample consisted of small particles irregularly shaped to form like aggregates. β-TCP synthesized using molarity ratio of 0.6M CaO: 0.4M H3PO4 had characteristics resembling β-TCP (Sigma-Aldrich).
Observation of new bone penetration into titanium rods with various thread pitch Putri, Tansza Setiana; Eddy, Eddy; Rinanti, Astri; Ishikawa, Kunio
Majalah Kedokteran Gigi Indonesia Vol 10, No 1 (2024): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.93519

Abstract

Titanium is a gold standard material in dental implant treatment due to its biocompatibility and excellent mechanical strength. However, titanium has no bioactivity and osteoconductivity. This has led to studies to develop the osteoconductivity by modifying the surface morphology, such as the thread pitch, which affect the implant stability and bone formation around the implant. This study aims to evaluate the effect of various size of gaps (equivalent to thread pitch) on the bone formation in titanium rods implantation. Initially, titanium rods were cut with different blade sizes: 0.2, 0.3, and 0.4 mm. The gaps were equivalent to dental implant thread pitch. Titanium rods were implanted in the rat’s femur and inserted into the bone marrow. After 2 and 4 weeks of implantation, the rats were euthanized and the implanted femur were extracted. The femurs were resin-embedded and cut into 1-mm thickness. The specimens were observed by backscattered SEM. Two weeks after implantation, new bone started to form and penetrated the pitch. In the wider gaps, the bone penetration was found to be particularly high, and vice versa. After 4 weeks, the new bone formation was greater compared to 2 weeks of implantation, and more bone penetration was observed in the wider pitch. This study is an observational research with qualitative reading of the backscattered SEM images. In conclusion, wider pitch could increase osseointegration by providing larger space for bone formation.
Degradation of chitosan–gelatin and chitosan–gelatin–β-tricalcium phosphate scaffolds Putri, Tansza Setiana; Pratiwi, Deviyanti; Margaretta, Dewi Liliany; Tjandrawinata, Rosalina; Shariff, Khairul Anuar
Dental Journal Vol. 57 No. 2 (2024): June
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v57.i2.p87-90

Abstract

Background: Fabrication of the composite scaffold was carried out by combining chitosan, gelatin, and β-tricalcium phosphate (βTCP) derived from limestone. The extraction of βTCP was based on the abundance of limestone containing calcium carbonate, which can be a source of βTCP synthesis. Purpose: This study evaluates the degradation of the combination of chitosan–gelatin (ChG) and chitosan–gelatin–βTCP (ChG-βTCP) composite scaffolds. Methods: The freeze-drying method was used to obtain the composite scaffold, which was a mixture of chitosan, gelatin, and βTCP. Degradation was measured by immersing the samples in a simulated body fluid solution at 37°C for 3, 7, 14, and 21 days. For statistical analysis, one-way analysis of variance (ANOVA) and post hoc Fisher's least significant difference were performed. Results: The ChG scaffold shows better degradability than the ChG-βTCP scaffold. The ChG scaffold shows higher weight degradation than the ChG-βTCP scaffold up to 21 days. Conclusion: In conclusion, the scaffold containing βTCP has lower degradation than the ChG scaffold.
Co-Authors Dewi Liliany Margaretta, Dewi Liliany Dipankara, Jackson Eddy Eddy, Eddy Kesnatri, Manuel Romario Kunio Ishikawa Pratiwi, Deviyanti Rosalina Tjandrawinata Santoso, Hillary Aurenne Shariff, Khairul Anuar Thi, Bang Le