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Inhibition Activity of Garlic (Allium sativum) Skin Aqueous Extract on Mastitis Causing Microorganisms Syamsi, Afduha Nurus; Pratiwi, Meyta; Nugroho, Aras Prasetiyo
ANIMAL PRODUCTION Vol 21, No 1 (2019)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (317.846 KB) | DOI: 10.20884/1.jap.2019.21.1.673

Abstract

The study was aimed to identify the effectiveness of the inhibitory activity of garlic skin extract (GSE) with different concentrations on the growth of Staphylococcus aureus, Streptococcus mutants, Escherichia coli, and Candida albicans. The study used the Kirby-Bauer method in a completely randomized (CRD) design with five treatments (positive control, negative control, 5% GSE, 10% GSE, and 15% GSE) and three replicates. The extract was obtained through evaporation of garlic skin macerated with aquadest solvent. The data were subjected to ANOVA, continued with an Honestly Significant Difference (HSD) test. The results showed that GSE concentration (minimum 5-10%) was highly significant to inhibit the growth of mastitis-causing microorganisms.
Synchronization Protein-Energy Index of Various Forages for Dairy Livestock: an In Vitro Study Syamsi, Afduha Nurus; Widodo, Hermawan Setyo
ANIMAL PRODUCTION Vol. 22 No. 2 (2020)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with Animal Scientist Society of Indonesia (ISPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (861.105 KB) | DOI: 10.20884/1.jap.2020.22.2.34

Abstract

The present study inventoried the protein-energy synchronization (PES) index of various types of grass. The study was conducted using in vitro techniques with material 5 types of grass (elephant, king, dwarf elephant, guinea, and setaria) and rumen fluid of the Friesian Holstein (FH). The PES index is calculated based on the average degradation of g protein and Kg organic matter (OM) per hour at the time of observation of 2nd, 4th, 6th, 8th, 12th, 24th, 48th, and 72nd hours. The hourly degradation rates were analyzed by linear regression to obtain the average hourly degradation rate. The results were included in the PES index calculation formula and discussed descriptively. This study found that all five grass had almost uniform nutritional value. Regression analysis results show a different equation, but with a high coefficient of determination (> 90%) on all types of grass. The PES index obtained in elephant, king, dwarf elephant, guinea, and setaria grass are 0.72, 0.66, 0.69, 0.58, and 0.68. The study concluded that elephant grass has a PES index at a high level, while king, dwarf elephant, guinea, and setaria grass at medium level for compiling dairy rations.
Identification of Goats’ and Cows’ Milk Protein Profile in Banyumas Regency by Sodium Dedocyl Sulphate Gel Electrophoresis (Sds-Page) Widodo, Hermawan Setyo; Astuti, Triana Yuni; Soediarto, Pramono; Syamsi, Afduha Nurus
ANIMAL PRODUCTION Vol. 23 No. 1 (2021)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with Animal Scientist Society of Indonesia (ISPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2021.23.1.37

Abstract

Protein is one of the nutrient components in milk that is related to product quality. The components of milk protein are divided into casein alpha-s1, beta, alpha-s2, kappa, and whey fractions such as alpha lactalbumin and beta lactoglobulin. There are no existing data of milk protein fraction in dairy cow and goats in Banyumas Regency. This study aimed to determine the profile in form of protein fractions of cow and goat milk in Banyumas. Milk sample from fifty cows and thirty dairy goats was taken by random sampling in some areas. The milk protein profile was identified by the technique of sodium dodecyl sulphate gel electrophoresis (SDS-PAGE) and protein quantity prediction by software. The data obtained were analyzed statistically by Mann-Whitney between cows and goats. The results were significantly different (p<0.05) between cows and goats in molecular weight of protein alpha-S1 casein (29.66 vs 33.37 kDa), alpha-S2 (27.76 vs 29.49 kDa), beta (24 , 48 vs 25.59 kDa) and beta lactoglobulin (15.75 vs 15.97 kDa). The quantity of casein alpha-S1 (7.88 vs 4.16 g/l), alpha-S2 (1.31 vs. 4.02 g/l), beta (8.74 vs 14.24 g/l), kappa (2.41 vs. 4.28 g/l) and alpha lactalbumin (0.91 vs 0.7 g / l) was significantly different (p <0.05) between cow's and goat's milk, respectively. In conclusion, milk protein profile of cows and goats in Banyumas Regency is different.