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Runtunuwu, Semuel David
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Agriculture, Biological Sciences & Forestry Environmental Science

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Growth of Potato Mericlone Shoots (Solanum tuberosum L.) At Several Concentrations of Kinetin And Coconut Water Tambun, Vistria; Lengkong, Edy Fredy; Runtunuwu, Semuel David; Supit, Paula C. H.; Tumewu, Pemmy; Inkiriwang, Annatje E. B.; Sompotan, Saartje; Liwu, Suzanne Laura; Doodoh, Beatrix; Mamarimbing, Rinny
Jurnal Agroekoteknologi Terapan Vol. 5 No. 1 (2024): EDISI JANUARI-JUNI 2024
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/jat.v5i1.51214

Abstract

This study aims to determine the effect and concentration of kinetin on potato mericlon shoots and to determine the best effect and concentration of coconut water on potato mericlon shoots. This research was conducted at the Plant Genetics Laboratory, Faculty of Agriculture, Sam Ratulangi University, Manado. This study used a completely randomized design (CRD) which consisted of seven treatments namely A0 (control/no treatment), A1 (Kinetin 0.5 ppm), A2 (Kinetin 1.5 ppm), A3 (Kinetin 1.5 ppm), A4 ( Coconut Water 5%), A5 (Coconut Water 7.5%), A6 (Coconut Water 10%). The variables observed were: shoot height, number of leaves, number of roots, and fresh weight. The results showed that the treatment of kinetin and young coconut water had a significant effect on the parameters of shoot height, namely A6 (6.74 cm), number of leaves, namely A1 (8.2 strands) and A6 (8.2 strands), number of roots, namely A3 (11 .3 units) and a wet weight of A3 (0.4591 gram). In this study, a kinetin concentration of 0.5 ppm was good for increasing the number of leaves, and a concentration of 10% coconut water was good for increasing shoot height. Keywords: Potato, Tissue Culture, PGR, Kinetin, Coconut Water. Abstrak Penelitian ini bertujuan untuk mengetahui pengaruh dan konsentrasi kinetin terhadap tunas meriklon kentang dan untuk mengetahui pengaruh dan konsentrasi terbaik air kelapa terhadap tunas meriklon kentangPenelitian ini dilaksanakan di Laboratorium Genetika Tanaman, Fakultas Pertanian, Universitas Sam Ratulangi Manado. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari tujuh perlakuan yaitu A0 (kontrol/tanpa perlakuan), A1 (Kinetin 0,5 ppm), A2 (Kinetin 1 ppm), A3 (Kinetin 1,5 ppm), A4 (Air Kelapa 5%), A5 (Air Kelapa 7,5%), A6 (Air Kelapa 10%). Variabel yang diamati adalah: tinggi tunas, jumlah daun, jumlah akar, dan berat basah. Hasil penelitian menunjukan pemberian perlakuan kinetin dan air kelapa muda berpengaruh nyata terhadap parameter tinggi tunas yaitu A6 (6,74 cm), jumlah daun yaitu A1 (8,2 helai) dan A6 (8,2 helai), jumlah akar yaitu A3 (11,3 unit) dan berat basah yaitu A3 (0.4591 gram). Pada penelitian ini, konsentrasi kinetin 0,5 ppm sudah baik untuk peningkatan jumlah daun, dan konsentrasi air kelapa 10% sudah baik untuk meningkatkan tinggi tunas. Kata kunci: Kentang, Kultur Jaringan, ZPT, Kinetin, Air Kelapa
Interaction of PGR Paclobutrazol and Nitrogen Nutrient on Growth and Yield of Potato (Solanum tuberosum L.) Granola L. Variety Darus, Deisire N. S.; Runtunuwu, Semuel David; Lengkong, Edy Fredy; Tulung, Stella
Jurnal Agroekoteknologi Terapan Vol. 5 No. 1 (2024): EDISI JANUARI-JUNI 2024
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/jat.v5i1.55262

Abstract

This study aims to analyze the interaction between paclobutrazol (PBZ) with Nitrogen (N) nutrients on the growth and yield of potato (Solanum tuberosum L.) var. Granola L. It was conducted in Mokobang Village, Modoinding District, South Minahasa Regency. This research was carried out in the form of field experiments and laboratory analysis. The treatment consisted of two factors: A. PBZ at doses: 1) 3 kg PBZ/ha, 2) 3.5 kg PBZ/ha, and 3) 4 kg PBZ/ha. B. Nitrogen nutrient at doses: 1) 200 kg N/ha, 2) 250 kg N/ha, 3) 300 kg N/ha. The placement used RGD and each treatment was repeated 3 times, there were 27 combinations (3 x 3 x 3). The results showed an interaction between the growth and yield of potato plants. Generally, higher doses of PBZ and N increased the growth of plant height, number of leaves, number of branches, leaf wet weight and dry weight, stem wet weight and dry weight, root wet weight and dry weight, plant wet weight and dry weight, and leaf chlorophyll content and tuber yield. The interaction of PBZ and N increased the tuber yield of potato plants per hectare. The highest tuber productivity resulted from the treatment of 4 kg PBZ and 200 kg N/ha (P3N1) at 37.9 tons/ha, while the lowest productivity resulted from the combination of 3 kg PBZ and 250 kg N/ha (P1N2) at 27.3 tons/ha. Keywords: Interaction, Paclobutrazol (PBZ), Nitrogen (N), Solanum tuberosum L. var. Granola L. Abstrak Penelitian ini bertujuan untuk menganalisis interaksi antara paclobutrazol (PBZ) dengan hara Nitrogen (N) terhadap pertumbuhan dan hasil kentang (Solanum tuberosum L.) var. Granola L. Dilaksanakan di Desa Mokobang Kecamatan Modoinding Kabupaten Minahasa Selatan. Penelitian ini dilaksanakan dalam bentuk percobaan lapangan dan analisis laboratorium. Perlakuan terdiri dari dua faktor yaitu: A. PBZ diberikan pada umur 6 MST, dengan dosis: 1) 3 kg PBZ/ha, 2) 3,5 kg PBZ/ha dan 3) 4 kg PBZ/ha. B. pupuk N diberikan pada umur 4 dan 6 MST, dengan dosis: 1) 200 kg N/ha, 2) 250 kg N/ha, 3) 300 kg N/ha. Penempatan menggunakan RAK dan setiap perlakuan diulang 3 kali, terdapat 27 kombinasi (3 x 3 x 3). Hasil penelitian menunjukkan adanya interaksi terhadap pertumbuhan dan hasil tanaman kentang. Umumnya, makin tinggi dosis PBZ dan N meningkatkan pertumbuhan tinggi tanaman, jumlah daun, jumlah cabang, berat basah dan berat kering daun, berat basah dan berat kering batang, berat basah dan berat kering akar, berat basah dan berat kering tanaman, kandungan klorofil daun, dan hasil umbi. Interaksi PBZ dan N meningkatkan hasil umbi tanaman kentang per hektar. Produktivitas umbi tertinggi dihasilkan dari perlakuan 4 kg PBZ dan 200 kg N/ha (P3N1) yaitu 37.9 ton/ha, sedangkan produktivitas terendah dihasilkan dari kombinasi 3 kg PBZ dan 250 kg N/ha (P1N2) yaitu 27.3 ton/ha. Kata kunci: Interaksi, Paclobutrazol (PBZ), Nitrogen (N), Solanum tuberosum L. var. Granola L.
Co-Authors Darus, Deisire N. S. Doodoh, Beatrix Inkiriwang, Annatje E. B. Lengkong, Edy Fredy Liwu, Suzanne Laura Pemmy Tumewu Rinny Mamarimbing saartje Sompotan Stella M.T. Tulung Supit, Paula C. H. Tambun, Vistria