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All Journal HAYATI Journal of Biosciences Sainmatika: Jurnal Ilmiah Matematika dan Ilmu Pengetahuan Alam
Hermansyah, Hermansyah
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, Sriwijaya University
Agriculture, Biological Sciences & Forestry Chemistry Earth & Planetary Sciences Mathematics Physics

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KONSTRUKSI DOUBLE DISRUPTANT RAGI Saccharomyces cerevisiae DENGAN METODE PERSILANGAN-TETRAD ANALISIS Hermansyah, Hermansyah
Sainmatika: Jurnal Ilmiah Matematika dan Ilmu Pengetahuan Alam Sainmatika Volume 7 No. 2 Desember 2010
Publisher : Faculty of Mathematics and Natural Science, Universitas PGRI Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31851/sainmatika.v7i2.12

Abstract

ABSTRAKFungsi redundan gen-gen ragi Saccharomyces cerevisiae dapat dipelajari melalui fenotip yang ditimbulkan ketika dua gen di rusak sekaligus. Konstruksi double disruptant protein fosfatase ptc2∆ msg5∆ dapat dilakukan dengan metodepersilangan-tetrad analisis. Single disruptant ptc2∆ dan msg5∆ disilangkan, kemudian dilakukan persilangan kembali antara ptc2∆ msg5∆ dengan msg5∆ dan dilakukan tetrad analysis. Hasil penelitian menunjuKkan bahwa hasil persilangan kembali tersebut menghasilkan pola segregasi 2:2 pada media sintetik media tanpa histidin antara ptc2∆ msg5∆ dengan msg5∆ . Hal ini mengindikasikan bahwa konstruksi double disruptant ptc2∆ msg5∆ berhasil dengan baik.Kata kunci: Saccharomyces cerevisiae, tetrad analisis, segregasi
Intracellular Ca2+ Regulation in Calcium Sensitive Phenotype of Saccharomyces cerevisiae . HERMANSYAH
HAYATI Journal of Biosciences Vol. 17 No. 1 (2010): March 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (88.721 KB) | DOI: 10.4308/hjb.17.1.1

Abstract

Intracellular cytosolic Ca2+ concentration accumulation plays an essential information in Saccharomyces cerevisiae i.e. to explain cellular mechanism of Ca2+ sensitive phenotype. Disruption both S. cerevisiae PPase PTP2 and MSG5 genes showed an inhibited growth in the presence of Ca2+. On the other hand, by using Luminocounter with apoaequorin system, a method based upon luminescent photoprotein aequorin, intracellular Ca2+ concentration was accumulated as a consequence of calcium sensitive phenotype of S. cerevisiae. This fact indicated that PPase  ptp2D and msg5D were involved in intracellular Ca2+ transport in addition their already known pathways i.e Mitogen Activated Protein Kinase cell wall integrity pathway, high osmolarity glycerol (HOG) pathway, and pheromone response FUS3 pathway.
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