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All Journal The Indonesian Biomedical Journal Jurnal Kreativitas PKM Jurnal Kedokteran Gigi Terpadu Jurnal Abdimas Kesehatan Terpadu
Dewi Ranggaini
Department Of Physiology, Division Of Oral Biology, Faculty Of Dentistry, Universitas Trisakti, Jl. Kyai Tapa No. 260, Jakarta 11440
Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Journal : Jurnal Kedokteran Gigi Terpadu

 1 
Nyeri Orofasial Sebagai Salah Satu Nyeri Alih Dari Iskemia Miokardium Monica Dewi Ranggaini
Jurnal Kedokteran Gigi Terpadu Vol. 2 No. 1 (2020): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (633.052 KB) | DOI: 10.25105/jkgt.v2i1.7528

Abstract

Referred pain on the orofacial structures can be diagnostically challenging for the dentists. Dental pain, which is the most common pain in the orofacial structures, is commonly  resolved with satisfactory result, However, some dental pain can have a non-odontogenic characteristics which originate from myocardial ischemia. Myocardial ischemia symptoms may be presented as angina pectoris specific pain, but also as trigeminal pain, with or without angina pectoris symptoms. When this occurred, an improper diagnostic can lead to unnecessary dental treatment and delayed of myocardial ischemia treatment which can lead to myocardium infarct.
Aktivitas antioksidan ekstrak etanol rimpang curcuma xanthorrhiza Roxb. dan asam askorbat (Dengan metode DPPH, FRAP, dan H2O2) Shannon Winnie Susanto; Monica Dewi Ranggaini
Jurnal Kedokteran Gigi Terpadu Vol. 4 No. 1 (2022): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (661.888 KB)

Abstract

Background: C. xanthorrhiza Roxb. contains chemical compounds that beneficial as antioxidant. Extraction using ethanol 70% is required to obtain these compounds. The antioxidant activity is assessed by using DPPH, FRAP, and H2O2. Parameter to interpret the value is IC50 and the value is compared to absorbic acid. Purpose: To find out the comparison of antioxidant activity between C. xanthorrhiza Roxb. extract in ethanol 70% solvent and absorbic acid using DPPH, FRAP, and H2O2 methods. Method: Using laboratory research method. Simplicia of C. xanthorrhiza Roxb. was extracted using maceration method in 70% ethanol. The antioxidant activity was tested in the sample and the absorbic acid. The samples were incubated and measured at  = 520 nm for DPPH,  = 593 nm for FRAP, and  = 510 nm for H2O2. Result: The IC50 value of C. xanthorrhiza Roxb. extract was 194,74 g/mL DPPH, 49.69 g/mL FRAP, and 166,75 g/mL H2O2 , and for absorbic acid was 0,29 g/mL DPPH, 2,78 g/mL FRAP, and 0,55 g/mL H2O2. Conclusion: FRAP method is proven to be the strongest method for measuring antioxidant activity in C. xanthorrhiza Roxb. among the other methods.
Aktivitas antioksidan ekstrak etanol rimpang curcuma xanthorrhiza roxb. Dan asam askorbat (Dengan Metode DPPH, ABTS, Dan NO) Olivia Amanda Suwardi; Monica Dewi Ranggaini
Jurnal Kedokteran Gigi Terpadu Vol. 4 No. 1 (2022): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (953.146 KB)

Abstract

Background: Antioxidants are very important to prevent degenerative diseases. Natural ingredients used as natural antioxidants are C. xanthorrhiza Roxb. Purpose: To compare antioxidant activity between ethanol extract C. xanthorrhiza Roxb. and ascorbic acid with DPPH, ABTS, and NO methods. Methods: Experimental laboratory research. C. xanthorrhiza Roxb. was extracted by maceration method. In the DPPH, ABTS, and NO methods, C. xanthorrhiza Roxb. and ascorbic acid were incubated. Then the absorbance was measured at a wavelength 517 nm for the DPPH method, 745 nm for the ABTS method, and 516 nm for NO method. Results: In DPPH method, IC50 of ethanol extract C. xanthorrhiza Roxb. was 194.74 g/mL and 0.29 g/mL for ascorbic acid, in ABTS method IC50 of ethanol extract C. xanthorrhiza Roxb. was 80.04 g/mL and 2.49 g/mL for ascorbic acid, in NO method, IC50 of ethanol extract C. xanthorrhiza Roxb. was 164.11 g/mL and 0.54 g/mL for ascorbic acid. Conclusion: In DPPH and NO methods, C. xanthorrhiza Roxb. has weak antioxidant activity and in ABTS method has strong antioxidant activity. In DPPH, ABTS, and NO methods, ascorbic acid has very strong antioxidant activity.
Efek sitotoksisitas ekstrak etanol 70% curcuma xanthorrhiza roxb. Terhadap sel raw 264.7 yang diinduksi lipopolisakarida "lps" (Laporan Penelitian) Fika Alifiana; Monica Dewi Ranggaini; Johni Halim
Jurnal Kedokteran Gigi Terpadu Vol. 4 No. 2 (2022): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jkgt.v4i2.15490

Abstract

Background: Curcuma xanthorrhiza Roxb. has bioactive compounds that are beneficial as anti-inflammatory. To get these compounds need to be extracted. Ethanol 70% can increase the solubility of both polar and non-polar compounds. RAW 264.7 cells with LPS induction cause an inflammatory state due to the active pro-inflammatory cytokines. To suppress the development of inflammation, a cytotoxicity test can be carried out to see the toxic nature of the extract against inflamed cells. Goals: To determine the cytotoxicity effect of 70% ethanol extract of C. xanthorrhiza on LPS-induced RAW 264.7 cells. Methods: In vitro laboratory experimental research. Preparation of extracts of C. xanthorrhiza Roxb. carried out with 70% ethanol then made concentrations of 200, 100, 50, 25, 12.5, 6.25, and 3.125 g/mL. Cytotoxicity was tested using the MTS. Untreated culture medium was used as a negative control and a positive control of diclofenac sodium. Then it was incubated for 24 hours at 37oC. After 24 hours of incubation, MTS was added to the plate and incubated again for 3 hours. The results were read using spectrophotometry. The resulting absorption is equivalent to living cells. The research data were analyzed using one-way ANOVA and Dunnet T3 tests. Results: Extracts of C. xanthorrhiza with concentrations of 200 and 100 g/mL were weakly cytotoxic and at concentrations of 50, 25, 12.5, 6.25, and 3.125 g/mL showed non-toxic effects on LPS-induced RAW 264.7 cells. Based on the IC50, C. xanthorrhiza Roxb. extract had a strong cytotoxic effect on LPS-induced RAW 264.7 cells. Conclusion: C. xanthorrhiza extract was toxic to LPS-induced RAW 264 cells.
Pengaruh ekstrak biji melinjo terhadap viabilitas dan apoptosis sel hsc-3 (Laporan Penelitian) Monica Dewi Ranggaini; Johni Halim; Richard Tridarmawan
Jurnal Kedokteran Gigi Terpadu Vol. 4 No. 2 (2022): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jkgt.v4i2.15525

Abstract

Background: Squamous cell carcinoma (SCC) is the most common form of oral cancer. SCC treatment generally uses surgical procedures, chemotherapy, etc. Currently there are more than 60% of anticancer compounds obtained from natural ingredients, one of which is Gnetum gnemon L. (G. gnemon L.) or commonly called melinjo. Objective: To determine whether the G. gnemon L. seed ethanolic extract with concentrations of 1, 10, and 100 µg/mL could reduce viability and induce apoptosis in the HSC-3 cell line. Methods: Laboratory experimental research (in vitro) was conducted using the cell line HSC-3. Cells were treated with G. gnemon L. seed ethanolic extract with concentrations of 1, 10, and 100 µg/mL for 24 hours. Ethanol 70% was used as solvent and negative control. Doxorubicin was used as a positive control. The treated cells were analyzed using MTT assay and flow cytometry to examine changes in cell viability and apoptosis that occurred. Results: G. gnemon L. seed ethanolic extract with concentrations of 1, 10, and 100 µg/mL could significantly reduce viability and induce apoptosis in the HSC-3 cell line (p<0.05). The ability to reduce viability increased with the increase in extract concentration as many as 8,169 cells, 5,789 cells, and 3,068 cells and also induces apoptosis by 8.33 ± 0.93%, 16.55 ± 1.51%, and 28.73 ± 0.89% of cells tested sequentially. Conclusion: G. gnemon L. seed ethanolic extract with concentrations of 1, 10, and 100 µg/mL for 24 hours was able to reduce viability and induce apoptosis in the HSC-3 cell line.
Aktivitas Antioksidan Ekstrak Etanol Bunga Clitoria ternatea L. Dengan Senyawa Antioksidan (Antosianin dan Mirisetin) Monica Dewi Ranggaini; Johni Halim; Intan Paramitha Kumaladevi
Jurnal Kedokteran Gigi Terpadu Vol. 5 No. 1 (2023): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jkgt.v5i1.16762

Abstract

Background: The COVID-19 pandemic is impacting a large number of populations. Patients with cancer infected with this virus require a longer treatment. Cancer occurs due to oxidative stress which creates an imbalance between the production of reactive oxygen species (ROS) and the amount of antioxidants in the body. Antioxidants are needed to balance ROS and increase the body's resistance to infection and disease. Clitoria ternatea L. is known to be one of the natural ingredients that has the potential as a natural antioxidant. It contains active compounds, such as anthocyanin and myricetin which act as antioxidants. Purpose: To find out the comparison of antioxidant activity C. ternatea L. extract in ethanol 70% solvent with the antioxidant compounds of anthocyanin and myricetin. Method: Research with laboratory experimental methods. C. ternatea L. simplicia was extracted using maceration method in 70% ethanol solvent. Antioxidant activity test using the 2,2-diphenyl-l-picrylhydrazyl (DPPH) method was carried out on samples of C. ternatea L. ethanol extract, anthocyanin and myricetin antioxidant compounds with a wavelength of 517 nm to measure their absorbance using a microplate reader. Result: The IC50 value of the ethanol extract of C. ternatea L. was 52.40 mg/mL, the antioxidant compound anthocyanin was 27.68 mg/mL and the antioxidant compound myricetin was 4.89 mg/mL. Conclusion: The antioxidant compound myricetin has very strong antioxidant activity compared to the ethanol extract of C. ternatea L. and the antioxidant compounds of anthocyanins.
Aktivitas Antioksidan dengan Metode DPPH dan ABTS Terhadap Ekstrak Etanol Daun Amaranthus hybridus L. Dewi Ranggaini, Monica; Halim, Johni; Aurelia Tjoe, Michelle
Jurnal Kedokteran Gigi Terpadu Vol. 6 No. 1 (2024): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jkgt.v6i1.20891

Abstract

Background: Cancer, a degenerative disease, arises from free radicals in the body, which can be counteracted by antioxidants. Amaranthus hybridus L., a vegetable rich in antioxidant compounds like flavonoids, requires extraction to harness these compounds. This extraction uses the maceration technique with 70% ethanol. Antioxidant activity is tested using methods like DPPH and ABTS assays, with the IC50 value indicating effectiveness. Purpose: To determine the difference in antioxidant activity of A. hybridus L. leaves ethanol extract with DPPH and ABTS methods. Method: Laboratory experimental research. Simplicia leaves of A. hybridus L. in 70% ethanol solvent were extracted using maseration method. The antioxidant activity was tested in the sample and the ascorbic acid using DPPH and ABTS methods at wavelenghts 517 nm and 745 nm. Sample absorbance was measured using microplate reader. Result: IC50 values of A. hybridus L. leaves ethanol extract with DPPH and ABTS methods were 54,99 µg/mL and 71,45 µg/mL, for ascorbic acid using DPPH and ABTS methods were 0,62 µg/mL and 3.13 µg/mL. Conclusion: The antioxidant activity of A. hybridus L. leaves ethanol extract with DPPH method has stronger antioxidant than ABTS method.
Co-Authors Alifah Evi Scania Annisaa Putri Ariyani Aurelia Tjoe, Michelle Bernard O. Iskandar Didi Nugroho Santosa Ferry Sandra Fika Alifiana Halim, Johni Intan Paramitha Kumaladevi Johni Halim Johni Halim Joko Kusnoto Kyung Hoon Lee Laifa Annisa Hendarmin Melanie Sadono Djamil Melanie Sadono Djamil Nurrani Mustika Dewi Olivia Amanda Suwardi Ria Aryani Hayuningtyas Richard Tridarmawan Richentya Feiby Salim Shannon Winnie Susanto Solachuddin Jauhari Arief Ichwan Tiffany Pang Wita Anggraini Wiwiek Poedjiastoeti, Wiwiek Yayuk Yuliarsi