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All Journal Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan
M. Hasbi, M. Hasbi
Dosen Program Studi Pendidikan Matematika FKIP Unsyiah
Earth & Planetary Sciences

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Isolation And Identification Of Biosurfactant Producing Bacteria In Sediments From The Coastal Area Of Mengkapan Village, Sungai Apit Distict, Siak Regency, Province Of Riau Kristina M, Martha Novia; M. Hasbi, M. Hasbi; Purwanto, Eko
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTThe activities of petroleum drilling, ferry ports and leakages of underwater pipeline caused oil pollution in the coastal area of the Mengkapan Village.The oil pollutant will be carried by the waves  and settles to the sediment and it may causing  genetic changing in the microorganisms present in the sediment. The presence of oil pollution may trigger gene mutation in specific bacteria and it may be able to produce biosurfactants for degrading the oil. A study aims to investigate of biosurfactant producing bacteria in the sediment of the  coastal area of Mengkapan Village was conducted from March to May 2018. There were 2 stations, in the petroleum drilling area (St1) and in the Tanjung Buton port (St2). Morphological and biochemical characeristics shown that there were 3 bacteria species, namely Bacillus sp, Proteus sp and Pseudomonas sp present. Emulsification Index of the Bacillus sp was 42,85%, the Pseudomonas sp was  53%,and  Proteus sp was 25%, indicating that all bacteria obtained were potencial for producing biosurfactant. Keyword : Oil pollutant ,Pseudomonas sp , Emulsification Index, Biodegradation
Isolation and Identification of Biosurfactant Producing Bacteria from Soil Aroundthe Gathering Station-Eor Plant PT. Bumi Siak Pusako – Pertamina Hulu Gultom, Susi Susanti; M. Hasbi, M. Hasbi; Purwanto, Eko
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTOil spilled polluted soil present around the Gathering Station (GS) pool of the PT. Bumi Siak Pusako – Pertamina Hulu. The presence of the oil may trigger gene mutation in specific bacteria in that area and they become able to produce biosurfactant for degrading the oil. A study aims to obtain the biosurfactant producing bacteria was conducted in April 2018. 100 grams of polluted soil samples were taken from the area around the Gathering Station pool. The soil was grinded and mixed with 40 ml Brain-Heart Infusion (BHI) media, and then was incubated for 1 x 24 hours. The bacteria were then grown using Blood Agar media and then was purified using MacConcey media. The pure bacteria were tested (biochemical and emulsification tests). Morphological and biochemical characteristics shown that there were 5 species of bacteria, namely Escheria coli, Pseudomonas aeroginosa, Bacillus careus, Proteus sp. and Klebsiella sp. Result of Emulsification index shown that all bacteria obtained were potencial for producing biosurfactant.Keywords: Oil spill, Escheria, Pseudomonas, Bacillus, Proteus, Klebsiella
Co-Authors Eko Purwanto Gultom, Susi Susanti Kristina M, Martha Novia