Article Per Year (5 Year)
p-Index From 2019 - 2024
1.399
P-Index
This Author published in this journals
All Journal Microbiology Indonesia Jurnal Tumbuhan Obat Indonesia Pharmaciana: Jurnal Kefarmasian Jurnal Ilmu Farmasi dan Farmasi Klinik (Journal of Pharmaceutical Science and Clinical Pharmacy) Pharmascience Pharmasipha Media Farmasi PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) JURNAL ILMU KEFARMASIAN INDONESIA Jurnal Kefarmasian Indonesia
Erika Indah Safitri
Universitas Wahid Hasyim
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

Published : 11 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : Pharmascience

 1 
Aktivitas Sitotoksik dan Induksi Apoptosis dari Ekstrak Etanol Kulit Apel Hijau (Pyrus malus L.) terhadap Sel MCF-7 Syahrul Iqbal Setiawan; Erika Indah Safitri; Devi Nisa Hidayati; Laili Nailul Muna
Jurnal Pharmascience Vol 8, No 2 (2021): Jurnal Pharmascience
Publisher : Program Studi Farmasi FMIPA Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v8i2.10729

Abstract

Ekstrak kulit apel hijau (Pyrus malus L.) mengandung senyawa Quercetin yang mampu menurunkan ekspresi Bcl-2. Penelitian ini bertujuan untuk mengetahui aktivitas sitotoksik dan induksi apoptosis ekstrak etanol kulit apel hijau (EEKAH) pada model sel kanker payudara MCF-7. Serbuk kulit apel hijau diekstraksi menggunakan pelarut etanol 70% dengan metode ultrasonik. Uji sitotoksik terhadap sel kanker payudara MCF-7 dengan metode MTT Assay pada konsentrasi EEKAH yaitu 31,25; 62,5; 125; 250; 500 dan 1000 µg/mL. Hasil uji sitotoksik dihitung nilai IC50menggunakan regresi linier. Pengamatan induksi apoptosis dengan konsentrasi ½ IC50(442,5 μg/ml) dan IC50 (885 μg/ml) menggunakan flowcytometry. Analisis data dianalisis menggunakan analisis statistik One Way Anova. Hasil penelitian menunjukan bahwa EEKAH memiliki aktivitas sitotoksik dengan nilai IC50 sebesar 885 µg/ml. Persentase rata-rata fase apoptosis total EEKAH pada konsentrasi ½ IC50sebesar 30,2% (p>0,05) dan IC50 sebesar 47,9% (p<0,05) dibandingkan kontrol sel sebesar 12,7%. EEKAH mampu menginduksi apoptosis terhadap sel MCF-7. Kata kunci: MCF-7, Pyrus malus L., Sitotoksisitas, Apoptosis  Green apple peel extract (Pyrus malus L.) contains Quercetin compound which decrease Bcl-2 expression. The aims of this study was to determined the cytotoxic activity and apoptosis induction of green apple peel ethanol extract (GASEE) on MCF-7 breast cancer cells. The green apple peel powder was extracted using 70% ethanol solvent by ultrasonic method. Cytotoxic test against MCF-7 breast cancer cells using the MTT Assay method at the GASEE concentration, namely 31.25; 62.5; 125; 250; 500 and 1000 µg/mL. The results of the cytotoxic test calculated the IC50 value using linear regression. Determination of apoptosis induction with a concentration of ½ IC50 (442.5 µg/mL) and IC50 (885 µg/mL) using flowcytometry. Data analysis was analyzed using statistical analysis One Way Anova. The results showed that GASEE has cytotoxic activity with an IC50 value of 885 µg/ml. Average percentage of total apoptosis phase are ½ IC50 concentration of 30.2% (p>0.05) and IC50 of 47.9% (p<0.05) than cell control of 12.6%. GASEE can apoptosis induced against MCF-7 cells.
Co-Authors Asri Melati Dewi AULIA EVERESTINA PUJAKA Ayu Shabrina Danang Yogo Wijaya Devi Nisa Hidayati Dewi Andini Kunti Mulangsri, Dewi Andini Kunti Dewi Listianingsih Dwi Ayu Mustikaningsih Dwi Lutvi Alviani Dwi Nadya Jayanthy Eko Wahyu Saputro Fera Elia Fita Ibrahim Arifin Intan Pratiwi Jeni Anggraini Laili Nailul Muna Masita Nur Amalia Putri Maulita Cut Nuria Misbahul Munir Raka Galih Pradana risha fillah fithria Risha Fillah Fitria Siska Maila Sari Sumantri Sumantri Syahrul Iqbal Setiawan Yance Anas Yunita Ayu Hapsari