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Muhsuryono, Muhsuryono
Politeknik Kelautan dan Perikanan Sorong
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Analisis Konsentrasi DNA dan Prediksi Protein Coral Hamid, Hamid; Supriatna, Iman; Gunaisah, Endang; Muhsuryono, Muhsuryono; Ismail, Ismail; Gofir, Abdul
Jurnal Airaha Vol 4 No 2: Desember 2015
Publisher : Politeknik Kelautan dan Perikanan Sorong

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Abstract

This study aimed to determine the concentration of DNA and proteins Coral predictions based on data from GenBank. DNA isolation technique Pocillopora sp. using tissue and blood DNeasy Kit (Qiagen). DNA concentrations were analyzed using a spectrophotometer and Corl protein prediction by comparing the primers used by the data GenBank (NCBI). DNA concentration of each sample varies between 5.0 ng/µl-20.8 ng/µl in the first purification and between 3.0 ng/µl - 8 ng/µl, where the highest concentration of DNA present in the sample HC2. DNA concentration and purity of samples obtained at HC2 and HC3. Prediction of protein based on data GeneBank is homologous with a NADH dehydrogenase in Coralliumkonojoi
Co-Authors Endang - Gunaisah Hamid Hamid I Gusti Wayan Murjana Yasa Iman Supriatna Yundari, Yundari