Garuda - Garba Rujukan Digital

The Correlation Between Total Calories And Protein Intake With Serum Albumin Level Of Post-Surgical Patients Saputra, Ivan; Arifin, Fransiscus; Suyono, Handi
JOURNAL OF WIDYA MEDIKA JUNIOR Vol 1, No 1 (2019)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (157.932 KB)

Introduction: Post-surgery diet is a set of foods that are given to patients after surgery. Nutritional management in post-surgical patients are often neglected despite the fact that patients require additional calories due to metabolic stress. Nutritional deficiency can impair wound healing since it is a process that requires protein to form collagen tissue. Aim: This study aims to determine the relationship between total calories and protein intake with serum albumin level of post-surgical patients. Methods: This study used an observational analytic design with cross-sectional approach. The sample was gathered using consecutive sampling technique. Study population were all post-surgical patients of Dr. Mohammad Soewandhie Hospital Surabaya aged 30-64 years whose medical records taken in June 2016. There are 32 respondents who met the inclusion criteria. Result: There was a significant correlation between the total calories intake with serum albumin level (r = 0.354; p = 0.047) and between total protein intake with serum albumin level (r = 0.545; p = 0.001). Conclusion: There is a positive correlation between total calories and protein intake with serum albumin level in post-surgical patients.

The Effect Of 10% And 30% Lavender Essential Oil Balm On Serum Cortisol Levels In Rats Given Stressor Putra, Christian JS; Suyono, Handi; Jong, F.X Himawan H.
JOURNAL OF WIDYA MEDIKA JUNIOR Vol 2, No 4 (2020): October
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwmj.v2i4.2734

Introduction: Indonesian Ministry of Health published Basic Health Research stated that the incidence of stress in Indonesia increased between 2013 and 2018. Untreated stress is a riskfactor for suicide and can cause the onset of depression. Stress associated with cortisol, thishormone has many functions in our body, such as increasing blood sugar levels, reducinginflammation, and suppressing the immune systemÂ One of the essential oils commonly used is English Lavender (Lavandula angustifolia). The lavender essential oil has many benefits, such as reduce anxiety, relieve pain, improve sleep quality, bactericidal, and repellent Purpose: This study aims to research the effectiveness of 10% and 30% lavender essential oil balm on serum cortisol levels in rats given stressor.Â Method: This study used 37 male rats randomly divided into four groups: negative control, positive control, 10% lavender balm, and 30% lavender balm. The forced swim test was given as the stressor every day for ten days, 20 days, and 30 days. The lavender oil balm was pplied to the back after the forced swim test. ELISA Kit measured the serum cortisol levels. Results: The results showed that 10% lavender essential oil balm significantly (p=0.007 and p=0.041) decreased serum cortisol levels compared to negative control and positive control group. However, there was no statistically significant difference in serum cortisol levels in the 30% lavender essential oil group. Furthermore, there was no significant difference in serum cortisol levels between 10 days, 20 days, and 30 days of the 10% and 30% lavender essential oil balm.Â Â Conclusion: The effectiveness of lavender essential oil balm to decrease the serum cortisol levels depends on the concentration and not depending on the duration of administration. 10% lavender essential oil balm lowers the serum cortisol levels more than 30% lavender ssential oil balm.

Effect Of Vetiver Essential Oil In 10% And 30% Concentration To The Cortisol Hormones Of Stressor-Induced Rats Willianto, Vincentius Michael; Suyono, Handi; Jong, F.X Himawan H.
JOURNAL OF WIDYA MEDIKA JUNIOR Vol 2, No 4 (2020): October
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwmj.v2i4.2739

Background: The number of people with mental health problems is still high in the world, especially depression. This incident is triggered by the stress of each individual on anongoing basis and failure to adapt. Stress regulation is a preventive measure to preventdepression in individuals. Currently, there are many developing uses of essential oils as antistress relaxation therapy. Vetiver essential oil is believed to reduce stress levels. Purpose: This study aimed to determine the effect of using vetiver essential oil with different concentrations and administration periods on changes in cortisol levels. Methods: This study used Rattus norvegicus experimental animals with the Forced Swim Test (FST) as a stressor. Vetiver essential oil concentrations of 10% and 30% were administered with the essential oil preparation given after giving stressor. Stress level in experimental animals was measured from the hormone cortisol in the blood. The method of measuring cortisol in experimental animals was carried out using the Enzyme-Linked Immuno Sorbent Assay (ELISA) method. Results: This study obtained significant results from the Kruskal Wallis statistical test ondecreasing cortisol levels in experimental animals by administering vetiver essential oil with10% concentration. Changes in the cortisol hormone in experimental animals and cortisollevels were not affected by the length of time of administration.

Terapi Oksigen Hiperbarik (TOHB) Pada Kasus Pasien Anak Tuli Mendadak Akibat Mumps Handi Suyono
JURNAL WIDYA MEDIKA Vol 4, No 2 (2018)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Background One of rare mumps sequalaein pediatric is deafness. Many treatments have been applied to sudden deafness including HBOT. HBOT is still useful and give good result eventhough it was delayed. Subject and Method The boy, 13 years old, was suddenly deaf of right ear 2 months ago. He got oral steroids from ENT specialist. There was no improvement after 2 months medications and then stopped the steroids. He had mumps 1 month before sudden deafness. The audiometry test showed hearing decreased 30-60 dB on frequency 125-8000 Hz. He had allergic rhinitis and tonsillitis in the past medical history, and tonsillectomy 6 years ago. HBOT was given 2.4 ATA 1 hour per session per day for 56 sessions (8 weeks). There are no barotrauma during and after HBOT. Result He felt hearing improvement after 44 sessions. The audiometry test showed improvement 10-20 dB. Conclusion HBOT is usseful and can give clinical improvement eventhough it was delayed.

ALCOHOL-BASED ANTISEPTIC SOLUTIONS ARE INEFFECTIVE IN INHIBITING PATHOGENIC FUNGI Handi Suyono; Laura Wihanto
JURNAL WIDYA MEDIKA Vol 7, No 2 (2021): October
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v7i2.3515

Introduction: Breaking the chain of transmission of infection using an antiseptic solution is an easy, inexpensive and effective method. The incidence of fungal infection is one of the global health problems that lead to severe complications even death, especially in patients with immunosuppressed or immunocompromised conditions and is reported to be increasing. Although alcohol-based antiseptic solutions are frequently used to prevent transmission of pathogenic organisms, these methods are rarely evaluated. Aim: Determine the susceptibility of three pathogenic fungi against alcohol-based antiseptic solutions with and without hydrogen peroxide addition. Methods: To determine the inhibition of the antiseptic solution against fungi, the Kirby & Bauer test disc diffusion method was used. After the petri dishes were incubated at 37° C for 24 hours, the diameter of the inhibition zones were measured using a caliper. The phenol coefficient test was carried out to compare the efficacy of an antimicrobial agent in this case alcohol-based antiseptic solutions against phenol. The phenol coefficient was applied as a test against Salmonella typhi, with a minimum score of 1, using the method according to SNI 1842: 2019. Result: The inhibition zone on Candida albicans ATCC 102231, Aspergillus niger, and Cryptococcus neoformans ATCC 14116 for solution A contains ethanol 80% v / v, glycerin 8% v / v, sterile water add 100% and solution B contains ethanol 80% v / v, H2O2 0.15% v / v, glycerin 8% v / v, add 100 sterile water % all were less than 6 mm which were classified as resistant. The result of the phenol coefficient test for solution A and B were 0.3 and 0.4 , which less than 1, indicated the antiseptic solutions were less effective than phenol. Conclusion: Antiseptic ethanol solution and a combination of ethanol + H2O2 were ineffective inhibits of pathogenic fungal growth. Further studies are needed to form a more potent antiseptic solution in order to improve management of fungal infections prevention.

Oksigen Hiperbarik (OHB) Menyembuhkan Sel Melalui Spesies Oksigen Reaktif (SOR) Handi Suyono
JURNAL WIDYA MEDIKA Vol 4, No 2 (2018)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

In degenerative diseases, hypoxia is the fundamental mechanism. Hypoxia stimulates reactive oxygen species (ROS) production. ROS interacts with protein, lipid, DNA, and other cell’s components and cause damage. Hyperbaric oxygen (HBO) improves clinical outcomes but the exact mechanism is still debated. HBO reverses hypoxia state. HBO increases ROS production because of hyperoxia but it can heal the cell. HBO-induced ROS stimulates endogenous antioxidants, hypoxia-inducible factors, and heat shock proteins. ROS theoretically improves telomere length and telomerase activity. Superoxide is one of ROS which converted into hydrogen peroxide and it acts as signal transduction. ROS has positive outcomes in maintaining cell survival

THE EFFECTS OF PINUS MASSONIANA (PINE POLLEN) AS ADJUVANT THERAPY ON LEUKOCYTES, T LYMPHOCYTES, C-REACTIVE PROTEIN, AND D-DIMERS IN HOSPITALIZED COVID-19 PATIENTS Prettysun Ang Melow; Handi Suyono; Maria Magdalena Ano Djoka; Agata Christi Palupi; Steven Hermantoputra; Anton Hariadi; Yudita Wulandari; Sri Purwaningsih
JURNAL WIDYA MEDIKA Vol 8, No 1 (2022): April
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v8i1.3626

Introduction: SARS-CoV-2 infection has caused the Corona Virus Disease-19 (COVID-19) pandemic until present day. Pine pollen has been known and used as a medicine and has benefits for immunity. Aim: To determine the effects of Pinus massoniana (pine pollen) as an adjuvant on leukocytes count, T lymphocytes (CD4, CD8) count, plasma C-reactive protein level, plasma D-dimer level in hospitalized COVID-19 patients with moderate-severe clinical manifestation. Materials and Method: We used a double blind pretest-posttest control group design, with 2 groups, namely control (placebo) and treatment. The subjects were selected by simple random sampling, male and female patients aged 20-60 years. Peripheral blood samples were taken before and after treatment. Patients were given pine pollen or placebo 3 x 4 capsules (3 g / day) for 7 days. Data were analyzed by SPSS 23.0 t-test with significant value of p < 0.05. Results: The patients of treatment group were 16 people (13 male, 3 female), average age 46,25 years and control 13 people (10 male, 3 female), average age 47,92 years. The leukocytes count increased not significantly in the treatment group (p=0.499; 8.03%) while control increased significantly (p=0.027; 36.42%). The leukocytes count of control was 4,5 times greater than treatment group. The CD4 count increased significantly in the treatment group (p=0.004; 73.44%) and control (p=0.048; 28.97%), and the it was 2,5 times greater than control. The CD8 count increased significantly in the treatment group (p=0.005; 72.90%) and control (p=0.033; 34.66%), and it was 2,1 times greater than control. CRP levels were significantly reduced in the treatment group (p=0.001; 71.61%) and control (p=0.001; 78.13%). D-dimer levels decreased significantly in the treatment group (p=0.048), while the control increased but not significantly (p=0.200). The D-dimer level in the treatment group decreased by 37.93%, while the control increased by 43.70%. Conclusion: Further research is still needed to explore the effect of pine pollen on cellular immunity which in this case is T lymphocytes, especially CD4 and CD8, and hemostasis (coagulation) especially D-dimer. Pine pollen is beneficial for immune modulation in COVID-19 patients.

EFFECTS OF ETHANOL-BASED ANTISEPTIC SOLUTIONS AGAINST GRAM NEGATIVE BACTERIA Laura Wihanto; Handi Suyono
JURNAL WIDYA MEDIKA Vol 7, No 1 (2021): April
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v7i1.2833

Introduction : Gram-negative bacteria infections cause diseases namely skin infection until sepsis including nosocomial infection. Prevention by antiseptic application is the way to inhibit infection. Some antiseptic compounds that have been used show resistance according to some reports. Aim: Determine the efficacy of ethanol-based antiseptic solutions against Gram-negative bacteria Methods : Discs saturated with ethanol-based antiseptic solutions were affixed to Muller Hinton agar which had been smeared by Acinetobacter baumannii ATCC BAA-747, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumonia ATCC BAA-1706, Escherichia coli ATCC 25922, Salmonella sp. , and Proteus vulgaris ATCC 6380. The diameter of the inhibition zone was read after 24 hours. Solutions are considered sensitive if the zone of inhibition of growth diameter is more than 6 millimeters (Kirby-Bauer disc diffusion method). Result : Acinetobacter baumannii and Salmonella sp is sensitive to solution consisting of ethanol 80 % and ethanol 80 %+ H2O2 0,15% . Pseudomonas aeruginosa and Eschericia coli is sensitive only to solution consisting of ethanol 80 %+ H2O2 0,15% As for Klebsiella pneumonia and Proteus vulgaris, they are resistance to both solutions. Conclusion: The use of ethanol-based antiseptic solutions with or without H2O2 0.15% addition may not effectively eliminate all gram-negative bacteria from the environment. The addition of 0.15% H2O2 to the antiseptic solution showed a better barrier effect than the solution containing only 80% ethanol. Adding other additives needs to be investigated further to formulate a better antiseptic solution against Gram-negative bacteria.

The Role of Antiapoptotic Erythropoietin on Ultraviolet B-Induced Photodamaged Skin Through Inhibition of Sunburn Cells Handi Suyono; Kristian Sanjaya; Deby Susanti
Folia Medica Indonesiana Vol. 56 No. 2 (2020): June
Publisher : Universitas Airlangga

Ultraviolet light causes photodamaged skin leading to photoaging skin. Ultraviolet-B (UV-B) causes epidermal keratinocyte apoptosis, namely sunburn cell, through apoptotic intrinsic pathway. Erythropoietin (EPO) has a role in cytoprotection in various tissues but its role to epidermal skin is not clear yet. This study was designed pretest-posttest control group design. Thirty two male mice Mus musculus, strain Balbc, were divided into control and treatment group, 16 mice each group. All mice were exposured by UV-B light 16mJ/cm2, distance 30 cm, duration 90 seconds, for 3 consecutive days. Four mice each group were randomly sacrificed as pretest data. The control mice were given aquadest subcutaneous injection 0.1 mL, and treatment mice were given EPO subcutaneous injection 0.1 mL (100 IU/kg BW). UVB were given everyday with same protocol above. Treatments were given 4 times, interval 3 days. All mice were sacrificed to examine sunburn cells. Data were performed as mean ± SD and analyzed by t-test using SPSS 17.0 with significant value p<0.05. Sunburn cells of pretest control were 25.00 ± 4.85% and treatment group were 24.83 ± 5.15%. Sunburn cells significantly decreased (p=0.002) in treatment group (31.5 ± 9.39%) than control (50.83 ± 6.70%). UV-B causes sunburn cells formation. EPO inhibits apoptosis through increasing NO production and eNOS expression, inhibiting caspase and proinflammatory cytokines, and increasing antiapoptotic protein. EPO has a role in skin apoptosis inhibition which is shown by decreased sunburn cells

Effects of combination of alcohol and Cinnamomum burmannii essential oil against Klebsiella pneumoniae resistance Jennifer Anggraini Sasangka; Handi Suyono; Gladdy Lysias Waworuntu
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 55, No 1 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19106/JMedSci005501202302

Alcohol-based antiseptics are widely used in the COVID-19 pandemic to prevent the transmission of infections, including bacterial infections. However, bacterial resistance to the alcohol-based antiseptics is begun reported. Klebsiella pneumonia resistance is one of the bacterial resistances that is prioritized by the WHO to be overcome. Cinnamomum burmannii essential oil, containing cinnamaldehyde and eugenol, was investigated for antimicrobial activity. This study aimed to evaluate the synergistic effect of the combination of alcohol and C. burmannii essential oil in inhibiting bacterial growth. Ethanol 80% in a combination with C. burmannii essential oil at concentrations of 1, 2, and 3% v/v were evaluated against K. pneumoniae using the Kirby-Bauer disc diffusion method. Test was repeated three times in independent experimental. Inhibition zone diameter (IZD, mm) and antimicrobial index (AI, %) were determined and analyzed using Kruskal-Wallis test continued the Mann-Whitney test. The combination of ethanol and C. burmannii essential oil was sensitive to K. pneumoniae, meanwhile, ethanol 80% was not more sensitive. The IZD of the combination solution at 1, 2, and 3% concentration were 6.7±0.19, 9.0±0.58, and 11.0±1.15mm, respectively (p<0.05). The AI of the combination solution at concentrations of 1, 2, and 3% v/v were 7.04±2.04, 30.53±6.79, and 51.64±12.91%, respectively (p<0.05). In conclusion, the combination of ethanol 80% and C. burmannii essential oil active against K. pneumoniae which resistant to the ethanol.

Title

Found 10 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 10 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 10 Documents
Search