Pharmaciana: Jurnal Kefarmasian
Vol 9, No 1 (2019): Pharmaciana

Solvent concentration effect on total flavonoid and total phenolic contents of Averrhoa bilimbi leaf extract

Muhammad Ryan Radix Rahardhian (Stifar "Yayasan Pharmasi Semarang")
Bayu Tri Murti (Stifar "Yayasan Pharmasi Semarang")
Dyah Wigati (Stifar "Yayasan Pharmasi Semarang")
Ririn Suharsanti (Stifar "Yayasan Pharmasi Semarang")
Chintiana Nindya Putri (Faculty of Pharmacy, Universitas Ahmad Dahlan)



Article Info

Publish Date
23 May 2019

Abstract

Averrhoa bilimbi is one of the Indonesian indigenous plants containing phenolic and flavonoids which exhibit several ethnopharmacological effects i.e. antidiabetic, anti-microbial, anti-inflammatory, cytotoxic, anti-oxidant, antifertility, and antibacterial activities. However, the optimum solvent for extracting these compounds using percolation have not been reported, yet. This research was aimed to evaluate the various concentrations of ethanol solvents towards total phenolic (TPC) and total flavonoid content (TFC). Extraction was carried out using percolation technique with ethanol solvent ratio of 50%, 70%, and 96%. The phytochemical screening, thin layer chromatography, total flavonoids, and total phenolic levels were obtained from the concentrated extract. The TPC and TFC were determined using visible spectrophotometry method. Phytochemical screening study indicated that ethanolic extract contains classes of flavonoids, phenolic, alkaloid, saponins, and steroids. The TFC of ethanolic extract with 50%, 70%, and 96% solvent concentrations were investigated at 62.74, 64.81 and 59.1 mg RTE / g extract, respectively while the TPC were recorded as follows: 103.79; 119.47; 110.10 mg GAE / g extract. Hence, 70% ethanol were higher among others, and therefore remains as optimum solvent for the extraction of A. bilimbi leaves. 

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Journal Info

Abbrev

PHARMACIANA

Publisher

Subject

Medicine & Pharmacology

Description

Pharmaciana is a scientific journal published by the University of Ahmad Dahlan worked closely with Ikatan Apoteker Indonesia (IAI). Pharmaciana published three times a year, namely March, July and November. with ISSN 2088-4559 and e-ISSN 2477-0256. The article published in the Journal Pharmaciana ...