Jurnal Sains dan Teknologi Reaksi
Vol 3, No 2 (2005): Jurnal Sains dan Teknologi Reaksi

Affinity Precipitation Of Enzyme By Using Conjugated Ligand-Poly(Nipam) Polymer

Syaubari, Syaubari (Unknown)
M, Saari M (Unknown)
T.G, Chuah (Unknown)
G, Zulkafli (Unknown)
M, Ishak (Unknown)

Article Info

Publish Date
22 Jun 2016

Abstract

In this paper, preparation of N-isopropylacrylamide of varying transfer chain lengths was synthesized. p-Aminobenzamidine (PABA) was chemically linked to the carboxyl groups of these polymers to obtain thermoprecipitating affinity polymers. The polymer to which PABA was linked through a carboxyl groups exhibited increase with polymer containing various PABA. Investigations on experiment showed that this enhancement in trypsin binding by the polymers was due to the p-aminobenzamidines to microenvironmental effects. Recovery and specific activity of the trypsin recovered increased with the low molecular weight of polymer.Keywords: Ligand, p-aminobenzamidine, trypsin, recovery, affinity precipitation

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Journal Info
Jurnal Sains dan Teknologi Reaksi
Website

Abbrev

JSTR

Publisher

Politeknik Negeri Lhokseumawe

Subject

Chemical Engineering, Chemistry & Bioengineering Chemistry Education Engineering Materials Science & Nanotechnology

Description

Jurnal Sains dan Teknologi Reaksi atau boleh disingkat dengan nama JSTR, berfokus pada banyak Aspek Teknik Kimia, seperti: Teknik Reaksi Kimia, Teknik Kimia Lingkungan, Energi Fosil dan Terbarukan, serta Sintesis dan Pengolahan Material. ...