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T.G, Chuah
Politeknik Negeri Lhokseumawe
Chemical Engineering, Chemistry & Bioengineering Chemistry Education Engineering Materials Science & Nanotechnology

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Affinity Precipitation Of Enzyme By Using Conjugated Ligand-Poly(Nipam) Polymer Syaubari, Syaubari; M, Saari M; T.G, Chuah; G, Zulkafli; M, Ishak
Jurnal Sains dan Teknologi Reaksi Vol 3, No 2 (2005): Jurnal Sains dan Teknologi Reaksi
Publisher : Politeknik Negeri Lhokseumawe

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30811/jstr.v3i2.51

Abstract

In this paper, preparation of N-isopropylacrylamide of varying transfer chain lengths was synthesized. p-Aminobenzamidine (PABA) was chemically linked to the carboxyl groups of these polymers to obtain thermoprecipitating affinity polymers. The polymer to which PABA was linked through a carboxyl groups exhibited increase with polymer containing various PABA. Investigations on experiment showed that this enhancement in trypsin binding by the polymers was due to the p-aminobenzamidines to microenvironmental effects. Recovery and specific activity of the trypsin recovered increased with the low molecular weight of polymer.Keywords: Ligand, p-aminobenzamidine, trypsin, recovery, affinity precipitation
Co-Authors G, Zulkafli M, Ishak M, Saari M Syaubari Syaubari