BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan
Vol 5, No 1 (2017): JURNAL BIOTIK

PERBANDINGAN METODE EKSTRAKSI DNA PHENOL-CHLOROFORM DAN KIT EXTRACTION PADA SAPI ACEH DAN SAPI MADURA

Kamaliah Kamaliah (Program Studi Biologi Fakultas Sains dan Teknologi UIN Ar-Raniry Banda Aceh)

Article Info

Publish Date
01 Apr 2017

Abstract

DNA extract is the first phase of molecular research which really influences the quality of DNA isolate. The aim of this study was to find out the comparison of DNA extract by using Phenol-Chloroform and KIT extraction. The visualization of DNA isolate resulted from electrophoresis technique after leptin gen of Aceh’s cow and Madura’s cow were amplificated through in vitro (Polymerase Chain Reaction). There were not any techniques of DNA extraction which can provide excellence results as a whole. Phenol-Chloroform extraction method gives advantage for lower price, more sensitive in isolating DNA, and clear DNA ribbon. Meanwhile, KIT extraction method is easier, fast, and thick DNA produced.

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Journal Info
BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan
Website

Abbrev

biotik

Publisher

Universitas Islam Negeri Ar-Raniry Banda Aceh

Subject

Biochemistry, Genetics & Molecular Biology Education

Description

BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan publishes scientific articles in the field of biology education and technology. Furthermore, this journal bridges the gap between research and practice, providing information, ideas and insights, in addition to critical examinations of biology ...