Biospecies
Vol. 12 No. 1 (2019): Vol. 12 No. 1, Januari 2019

Jenis – jenis tumbuhan paku (Pteridofita) dari Hutan Universitas Riau, Provinsi Riaudan Pola Pita DNA berdasarkan Penanda DNA M13Primer

Nery Sofiyanti (Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Riau)
Mayta Novaliza Isda (Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Riau)

Article Info

Publish Date
02 Apr 2019

Abstract

University of Riau Forest, Riau Province is one of distribution area of ferns (Pteridophytes). This study aimed to identify fern species at University of Riau Forest and examined the DNA band pattern based on M13 DNA marker. Samples were collected from the field using exploration method and were then identified. DNA isolation was carried out using DNAeasy Plant Mini Kit (Qiagen), and amplified based on PCR method using DreamTaq Green PCR Master Mix (2X) (Thermo Scientific) with M13 DNA Marker. The inventorisation result showed a total of 26 fern species belong to 8 families were identified in this study. The electrophoresis result of PCR product gave the variaous DNA band pattern ranged from 200 to 2000 bp. DNA band pattern using M13 primer can be used to support the taxonomical data of fern taxa

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Journal Info
Biospecies
Website

Abbrev

biospecies

Publisher

Universitas Jambi

Subject

Agriculture, Biological Sciences & Forestry

Description

Biospecies merupakan media komunikasi ilmiah untuk kajian di bidang Biologi. Biospecies terbit dua kali setahun, sejak tahun 2008. Penerbitan dilakukan pada setiap bulan Januari dan ...