Buletin Veteriner Udayana
Vol. 11 No. 2 Agustus 2019

Variasi Genetik Lokus Mikrosatelit D7S1789 pada Populasi Monyet Ekor Panjang di Gunung Pusuk Lombok Barat

Febio Tomasini Marciano Meus (Praktisi Dokter Hewan di Kabupaten Manggarai Timur, Nusa Tenggara Timur, Indonesia 86571.)
I Nengah Wandia (Fakultas Kedokteran Hewan
Pusat Penelitian Satwa Primata LPPM Universitas Udayana, Jl. Raya Kampus Udayana, Bukit Jimbaran, Kuta Selatan, Badung, Bali, Indonesia 80361.)

I Ketut Suatha (Fakultas Kedokteran Hewan
Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali Indonesia 80234.)



Article Info

Publish Date
31 Aug 2019

Abstract

Long-tailed macaques (Macaca fascicularis) are Southeast Asian endemic animal with its behaviors and interactions form a protective cohesive group on its territory. Such patterns create distinctions that can be detected by microsatellite variations. This study revealed polymorphism of the D7S1789 microsatellite locus in a population of long-tailed macaques in Mount Pusuk West Lombok with the aim of knowing the genetic variation in the form of number of alleles, allele frequencies, heterozygosity and Hardy-Weinberg Equilibrium based on its allele’s distributing the Chi-square test. A total of 17 blood samples of long tailed macaque extracted used QIAamp DNA blood kit from QIAGEN to get DNA, DNA from locus D7S1789 microsatellite was replicated through 30 cycles of PCR technique, with annealing temperature 57°C. The alleles were raised by silver staining using 8% polyacrylamide gel. The results obtained two alleles with a frequency of 121 bp (0.794) and 130 bp (0.206), heterozygosity (0.337) and Hardy-Weinberg Equilibrium test (?2 count) was 0.422 (?2 table = 3.841; ? = 0.05; db = 1) which indicates a random mating population. It can be concluded that the D7S1789 microsatellite locus was polymorphic in the population of long-tailed monkeys in Mount Pusuk, West Lombok.

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