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All Journal Buletin Veteriner Udayana
Febio Tomasini Marciano Meus
Praktisi Dokter Hewan di Kabupaten Manggarai Timur, Nusa Tenggara Timur, Indonesia 86571.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Variasi Genetik Lokus Mikrosatelit D7S1789 pada Populasi Monyet Ekor Panjang di Gunung Pusuk Lombok Barat Febio Tomasini Marciano Meus; I Nengah Wandia; I Ketut Suatha
Buletin Veteriner Udayana Vol. 11 No. 2 Agustus 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.148 KB) | DOI: 10.24843/bulvet.2019.v11.i02.p11

Abstract

Long-tailed macaques (Macaca fascicularis) are Southeast Asian endemic animal with its behaviors and interactions form a protective cohesive group on its territory. Such patterns create distinctions that can be detected by microsatellite variations. This study revealed polymorphism of the D7S1789 microsatellite locus in a population of long-tailed macaques in Mount Pusuk West Lombok with the aim of knowing the genetic variation in the form of number of alleles, allele frequencies, heterozygosity and Hardy-Weinberg Equilibrium based on its allele’s distributing the Chi-square test. A total of 17 blood samples of long tailed macaque extracted used QIAamp DNA blood kit from QIAGEN to get DNA, DNA from locus D7S1789 microsatellite was replicated through 30 cycles of PCR technique, with annealing temperature 57°C. The alleles were raised by silver staining using 8% polyacrylamide gel. The results obtained two alleles with a frequency of 121 bp (0.794) and 130 bp (0.206), heterozygosity (0.337) and Hardy-Weinberg Equilibrium test (?2 count) was 0.422 (?2 table = 3.841; ? = 0.05; db = 1) which indicates a random mating population. It can be concluded that the D7S1789 microsatellite locus was polymorphic in the population of long-tailed monkeys in Mount Pusuk, West Lombok.
Co-Authors I Ketut Suatha i Nengah Wandia