This study aims were to determine the total number of bacteria at maturity level and farm locations. As many as 24 catlle fecal samples were examined using the casting method. This study used a Randomized Complete Block Design of 2 x 3 factorial consist of two farm locations (highlands and lowlands) and three levels of cattle maturity (calves, heifers, adult). The total bacteria were analyzed using Analysis of Variance and continued by Least Significant Difference Test. The results of the total number bacteria isolated from feces adult 724.6250 x 109 CFU/g higher than heifers 667.5000 x 109 CFU/g and calf 499.5000 x 109 CFU/g. Total number of bacteria isolated from feces of bali cattle from highland 629.4167 x 109 CFU/g is not showed significant different compare to bali cattle from lowland 631.6667 x 109 CFU/g.
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