JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI
Vol 7 No 2 (2019): Juni

PRODUKSI ENZIM SELULASE KASAR DARI BAKTERI SELULOLITIK

Monalisa Nababan (Mahasiswa Program Studi Teknologi Industri Pertanian, Fakultas Teknologi Pertanian, Unud)
Ida Bagus Wayan Gunam (Dosen Program Studi Teknologi Industri Pertanian, Fakultas Teknologi Pertanian, Unud)
I Made Mahaputra Wijaya (Dosen Program Studi Teknologi Industri Pertanian, Fakultas Teknologi Pertanian, Unud)



Article Info

Publish Date
21 May 2019

Abstract

The purpose of this research was to elucidate the potentials of cellulolytic bacteria isolates in producing crude cellulose enzymes and to determine the ability of exoglucanase enzymes and endoglucanase enzymes produced from selected cellulolytic bacteria isolates. Ten potential isolates from previous research were confirmed using 1% Carboxymethyl Cellulose (CMC) media with the addition of Congo red and four potential isolates were obtained which then crude cellulose production and enzyme activity tests were carried out using cellulose substrates such as Carboxymethyl Cellulose (CMC), and Filter Paper (FP) Whattman number 1. The exoglucanase enzyme activity was obtained between 0.069 IU/mL to 0.072 IU/mL. The activity test of endoglucanase enzyme was obtained between 0.069 IU / mL to 0.074 IU/mL. Cellulose degradation was obtained between 3.32% to 11.37%. Isolate that have the highest enzymatic activity and cellulose degradation ability was B2S8. Keywords: Cellulolytic bacteria, Cellulase enzyme, Enzyme activity, Congo red.

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Journal Info

Abbrev

jtip

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI adalah media publikasi ilmiah yang diterbitkan oleh Program Studi Teknologi Industri Pertanian, Fakultas Teknologi Pertanian-Universitas Udayana, berisikan artikel hasil-hasil penelitian, ulasan (review), opini ilmiah oleh mahasiswa, dosen, praktisi, dan ...