MARINA CHIMICA ACTA
Vol. 16 No. 1 (2015): Enzyme from Bacteria and Coral Age

PRODUCTION OF PROTEASE ENZYME FROM BACTERIA IN HOT SPRING OF SOUTH SULAWESI, Bacillus licheniformis HSA3-1a

Irwan R (Universitas Pattimura)
Hasnah Natsir (Chemistry Department, Faculty of Mathematics and Natural Sciences, Hasanuddin University)

Article Info

Publish Date
01 Apr 2015

Abstract

Bacillus licheniformis HSA3-1a is able to produce extracellular protease. The aim of this research was to find out its production optimum time, consentration of MnCl2, temperature and optimum pH, also the cofactor influence on activity protease from B. licheniformis HSA3-1a. The protease activity was tested with a modified method of Walter. Protease production time was optimum for 36 hours with an activity value of 0,394 U/mL, protein content was 132,35 mg/mL. The Optimum condition of protease enzyme were pH 7,0 and temperature 45 ⁰C with an activity value of 0,0463 U/mL. Protease activity could be inhibited by MnCl2 concentration of 0,01 - 0,03 M.

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Journal Info
MARINA CHIMICA ACTA
Website

Abbrev

mca

Publisher

Universitas Hasanuddin

Subject

Biochemistry, Genetics & Molecular Biology

Description

When the first time appeared in 1999, Marina Chimica Acta was introduced as trilingual journal meaning that we can accept to publish articles in any kind of three languages such as bahasa Indonesia, English, and French. Starting April 2017, however, the publication has been made in English only, no ...