Jurnal Perikanan Universitas Gadjah Mada
Vol 21, No 2 (2019)

Isolation of Cellulolytic Bacterium Staphylococcus sp. JC20 from the Intestine of Octopus (Octopus sp.) for Fish Probiotic Candidate

Indah Istiqomah (Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta)
Alim Isnansetyo (Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta)
Imelda Novita Atitus (Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta)
Ahmad Fauzi Rohman (Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta)



Article Info

Publish Date
30 Dec 2019

Abstract

Aim of this study was to isolate, characterize, and identify cellulolytic bacteria from the digestive tract of marine vertebrates and invertebrates as a candidate of fish probiotics. The bacteria were isolated from the digestive tract and grown on a cellulose agar plate. The bacteria were screened based on the cellulolytic activity, acid resistance, antagonist activity against fish pathogens, antibiotics sensitivity, ability to live in fish digestive tract and non-pathogenic test. Selected bacterium was identified molecularly, based on the 16S rDNA gene sequences, and phenotipically. A total of 14 bacteria demonstrated celulolitic index of 1.1-1.8. The bacteria with cellulolytic index of > 1.6 were screened by the selection criteria, resulted a selected strain, JC20 isolate which was isolated from the digestive tract of octopus (Octopus sp.). The selected bacterium was sensitive to antibiotics, resists to acidic environment, able to live in the fish digestive tract, and non-pathogen. Thus, the bacterium was potential for further characterization as fish probiotics candidate. Molecular and phenotypic identification revealed that JC20 isolate was Staphylococcus sp.

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Journal Info

Abbrev

jfs

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Description

Jurnal Perikanan Universitas Gadjah Mada are published to promote a critical review of the various investigative issues of interest in the field of fisheries between the researchers, academics, students and the general public, as a medium for communication, dissemination, and utilization of wider ...