Biotropika
Vol 3, No 2 (2015)

The Effect of Different Media Content on Protease Activity Bacillus subtilis

Siti Nurkasanah (Molecular Biology Laboratory)
Nashi Widodo (Unknown)

Article Info

Publish Date
29 Jun 2015

Abstract

ABSTRACT Enzymes were protein molecules that synthesized cells to accelerate biochemical reactions. One of the microorganisms that produce a protease was Bacillus subtilis. B. subtilis used in this research consisted of isolate 1 and isolate 2. Methods used include thequalitative assay of protease activity through theclear zone on skim milk agar and calculation of protease activity on production media such as NB (Nutrient Broth) and TSB (Tryptic Soy Broth). Based on the results revealed that the clear zonediameter isolate 2 better than the isolate 1 after 24hours and 48 hours incubation periods. It showed both isolates having the possibility in different strains but within a species. The highest protease activity calculation obtained from TSB production media respectively  0,14 Unit /ml unit and 0.12 Unit/ml. Keywords:B. subtilis, clear zone, protease, TSB

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Journal Info
Biotropika
Website

Abbrev

biotropika

Publisher

Universitas Brawijaya

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Description

Biotropika: Journal of Tropical Biology invites research articles, short communication, and reviews describing new findings/phenomena of biological sciences in tropical regions, specifically in the following subjects, but not limited to biotechnology, biodiversity, microbiology, botany, zoology, ...