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All Journal Biotropika Indonesian Journal of Cancer Chemoprevention Journal of Community Empowerment for Health Jurnal Abdi Insani Indonesian Journal of Cancer
Siti Nurkasanah
DIII Blood Bank Technology, STIKES Rajekwesi, Bojonegoro
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Public Health

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The Effect of Different Media Content on Protease Activity Bacillus subtilis Nurkasanah, Siti; Widodo, Nashi
Biotropika: Journal of Tropical Biology Vol 3, No 2 (2015)
Publisher : Biotropika: Journal of Tropical Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (94.648 KB)

Abstract

ABSTRACT Enzymes were protein molecules that synthesized cells to accelerate biochemical reactions. One of the microorganisms that produce a protease was Bacillus subtilis. B. subtilis used in this research consisted of isolate 1 and isolate 2. Methods used include thequalitative assay of protease activity through theclear zone on skim milk agar and calculation of protease activity on production media such as NB (Nutrient Broth) and TSB (Tryptic Soy Broth). Based on the results revealed that the clear zonediameter isolate 2 better than the isolate 1 after 24hours and 48 hours incubation periods. It showed both isolates having the possibility in different strains but within a species. The highest protease activity calculation obtained from TSB production media respectively  0,14 Unit /ml unit and 0.12 Unit/ml. Keywords:B. subtilis, clear zone, protease, TSB
The Effect of Different Media Content on Protease Activity Bacillus subtilis Siti Nurkasanah; Nashi Widodo
Biotropika: Journal of Tropical Biology Vol 3, No 2 (2015)
Publisher : University of Brawijaya

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Abstract

ABSTRACT Enzymes were protein molecules that synthesized cells to accelerate biochemical reactions. One of the microorganisms that produce a protease was Bacillus subtilis. B. subtilis used in this research consisted of isolate 1 and isolate 2. Methods used include thequalitative assay of protease activity through theclear zone on skim milk agar and calculation of protease activity on production media such as NB (Nutrient Broth) and TSB (Tryptic Soy Broth). Based on the results revealed that the clear zonediameter isolate 2 better than the isolate 1 after 24hours and 48 hours incubation periods. It showed both isolates having the possibility in different strains but within a species. The highest protease activity calculation obtained from TSB production media respectively  0,14 Unit /ml unit and 0.12 Unit/ml. Keywords:B. subtilis, clear zone, protease, TSB
The Effectiveness of Topical 5-fluorouracil Treatment on Mouse Skin Squamous Cell Precancerous Lesions through Caspase-3 Expression Siti Nurkasanah; Aida S.D. Hoemardani; Evlina Suzanna Sinuraya; Puspita Eka Wuyung
Indonesian Journal of Cancer Chemoprevention Vol 13, No 1 (2022)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev13iss1pp12-21

Abstract

Skin cancer is a disease that develops in the epidermis of the skin and can be invasive, such as squamous cell carcinoma (SCC). Early detection of squamous cell precancerous can prevent these lesions from progressing to invasive SCC and increase the effectiveness of therapy. 5-fluorouracil (5-FU) is an antimetabolite compound as a pyrimidine DNA/RNA antagonist molecule that induces cell apoptosis. The main objective of this study was to evaluate the effectiveness of the topical 5-FU cream (Dharmais NCH) compared to imiquimod 5% on apoptosis through the expression of caspase-3 in precancerous squamous cells of mouse skin induced by 7,12-dimethylbenzen[a]-anthracene (DMBA)/croton oil treatment. This research assess three differences concentration of 5-FU include 1%, 2%, and 5% on 24 wild type mouse divided into 6 groups including positive control (with carcinogenesis but without treatment), negative control (without treatment; normal), carcinogenesis with treatment 5-FU cream (1%, 2%, and 5%) or 5% imiquimod cream. Two-stages carcinogenesis induced by DMBA and followed by croton oil. The expression of caspase-3 was analyzed using immunohistochemistry. Statistical analysis was performed by one-way ANOVA using SPSS version 23. The induction of two-stages of carcinogenesis (weeks 1 to 10) caused papilloma lesions on the skin of mouse. Furthermore, 5-FU treatment for 4 weeks (weeks 11 to 14) showed a decrease in the cumulative number of papillomas (p<0.05) and immunohistochemical analysis showed caspase-3 expression on 5-FU treatments (1%, 2%, and 5%) was not significantly different from the imiquimod treatment (p>0.05). The apoptotic effect of 5-FU treatment on precancerous skin squamous cell lesions in mouse was not significantly different from the standard treatment using imiquimod. This suggests that 5-FU treatment has potential as a future therapy in squamous cell precancerous skin lesions.Keywords: 5-fluorouracil, caspase-3, squamous cell precancerous, skin, topical treatment.
PEMERIKSAAN GOLONGAN DARAH SISTEM ABO RHESUS PADA MAHASISWA STIKES RAJEKWESI BOJONEGORO Fatia Rizki Nuraini; Nina Difla Muflikhah; Siti Nurkasanah
Jurnal Abdi Insani Vol 9 No 2 (2022): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v9i2.566

Abstract

Blood Type is an important thing to every person to understand and can identify using a specific methods, blood typing. Blood typing refers to a specific pattern of reaction to testing antisera within a given system. Blood typing have several methods, the common method that usually used is Direct Slide Test. Over a period of time, our understanding on blood groups has evolved to encompass not only transfusion-related problems but also specific disease association with RBC surface antigens. This community service activity aimed to provide blood typing examination for students who unknown their blood type and strenghten the understanding about the important of Blood Type. This activity was held on December 20, 2021 in Stikes Rajekwesi Bojonegoro. This Community service applied an examination test to several students who still unknown their blood type. Examination of blood group used the A-B-O system and rhesus with the slide test method. The results of the blood group examination of 16 students showed that the highest results were blood group B as many as 6 people (37.5%) and the least blood group being O and AB with 3 people each (18.75). Rhesus examination showed that all students were rhesus positive (100%). Students who examinated during the programmes doesn’t have undestanding of the important of knowing their blood type. Students of Stikes Rajekwesi Bojonegoro get sufficient understanding about the important of knowing their blood type and the blood typing showed 37,5% of student are B type, 18,75% are O and AB type each, while 100% rhesus positive.
Expression of Ki67 in Precancerous Squamous Cell Skin Lesion of Mice Induced by DMBA Siti Nurkasanah; Aida S D Hoemardani; Evlina Suzanna Sinuraya
Indonesian Journal of Cancer Vol 13, No 2 (2019): June
Publisher : National Cancer Center - Dharmais Cancer Hospital

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1179.783 KB) | DOI: 10.33371/ijoc.v13i2.638

Abstract

Background: Squamous cell carcinoma (SCC) is an invasive skin cancer that is found in the human skin. SCC growth from the precancerous lesion is known as a wart or papilloma. Ki67 is a protein expressed by cells during proliferation. Cells with higher Ki67 expression showed abnormal regulation of apoptosis. 5-FU is an antimetabolite compound that works as a DNA/RNA pyrimidine antagonist molecule that can induce cell apoptosis. The main objective of this research is to evaluate the inhibition proliferation of precancerous squamous cell in skin lesion induced by DMBA/croton oil treated by using 5-FU cream topically compared to 5% imiquimod cream. Methods: This research assesses three different concentrations of 5-FU cream namely 1%, 2%, and 5% topically on 24 wild type mice divided into 6 groups of 4 each including positive control (with carcinogenesis but without treatment), negative control (without treatment ; normal), carcinogenesis with treatment 5-FU cream (1%, 2%, and 5%) or 5% imiquimod cream. The expression of Ki67 was analyzed using immunohistochemistry. Statistical analysis was performed by one-way ANOVA using SPSS version 23. Results: Based on this research, 5-FU cream treatment during 4 weeks (week 10-week 14) showed the decrease of cumulative number of papilloma (p<0.05) and immunohistochemistry analysis resulted in the lowest Ki67 expression in 5-FU 2% and 5% groups compared to 5% imiquimod cream (p<0.05). Conclusions: 5-FU cream treatment during 4 weeks showed a decrease of papilloma and Ki67 expression in 5-FU cream 2% and
Analysis of blood donors’ characteristics during third-peak COVID-19 in Bojonegoro Regency, Indonesia siti - nurkasanah
Journal of Community Empowerment for Health Vol 6, No 1 (2023): Vol 6, No 1 (2023)
Publisher : Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jcoemph.80232

Abstract

The third peak of Coronavirus disease 2019 (COVID-19) in Indonesia was recorded on February-March 2022 had an impact on voluntary blood donors at the Indonesian Red Cross (IRCS) Bojonegoro. The objective of this research was to analyze the blood donors’ characteristics during third-peak COVID-19 in Bojonegoro Regency. This research was descriptive analysis study using secondary blood donor data from February 22, 2022 to March 19, 2022 at IRCS Bojonegoro. The data was collected in donor selection process accordance the Indonesian and WHO blood donor selection guidelines. The average number per daily blood donors was tested using the Mann-Whitney-U test using IBM SPSS type 23 software with significancy p<0.05. The results showed there were 219 people (80.51%) on 22-28 February and 332 people (83.63%) on 1-19 March 2022 who approved to donate at IRCS Bojonegoro. There was a significant decrease in the average of daily male blood donors (p=0.01), average age group: 17-24 years (p=0.013), 35-44 years (p=0.002), and 45-54 years (p=0.030), hemoglobin (Hbl) levels on 16.1-17 g/dL (p=0.000), systolic blood pressure (SBP) <120 (p=0.03) and 130-139 (p= 0.02) and diastolic blood pressure (DBP) <80 (p=0.001), pulse rate (PR) 65-74 beats/minute (p=0.016), and blood type O+ (p=0.008). The characteristics of blood donors at the third peak of COVID-19 in Bojonegoro Regency decreased significantly in the number of male blood donors, age group, SBP, DBP, PR, and O+ blood type.
Co-Authors Aida S D Hoemardani Aida S.D. Hoemardani Evlina Suzanna Sinuraya Evlina Suzanna Sinuraya Fatia Rizki Nuraini Nashi Widodo Nina Difla Muflikhah Puspita Eka Wuyung